biostudies-literatureHe XDistinguished Young Scholars in DalianNatural Science Foundation of Liaoning ProvinceLiaoning Revitalization Talents Program1875https://www.ebi.ac.uk/biostudies/studies/S-EPMC12521673biostudies-literatureUnknown16(1)<h4>Backgrounds</h4>Pancreatic cancer is one of the most common malignancies in the gastrointestinal system, notorious for its high malignancy and low survival rate. Amentoflavone (AMF), an ingredient in various Traditional Chinese Medicines (TCM), possesses anticancer properties. However, the underlying mechanisms of its action remain unclear.  METHODS: Initially, through the application of network pharmacology and molecular docking methodologies, we elucidated the putative core targets and signaling pathways underlying the antineoplastic potential of amentoflavone. Subsequently, we corroborated the therapeutic efficacy and underlying mechanisms of amentoflavone within both in vitro pancreatic cancer cell models and in vivo animal models.<h4>Results</h4>The key targets of amentoflavone weDiscover oncologyNetwork pharmacology and experimental study on the inhibition of glycolysis by amentoflavone in pancreatic cancer.PMC12521673(2022RJ19XLYC19071132023010109-JH2/1013Choe HChen PAli THe XMa JZhang LZhao ZDing KWu XfalseNetwork pharmacology and experimental study on the inhibition of glycolysis by amentoflavone in pancreatic cancer.<h4>Backgrounds</h4>Pancreatic cancer is one of the most common malignancies in the gastrointestinal system, notorious for its high malignancy and low survival rate. Amentoflavone (AMF), an ingredient in various Traditional Chinese Medicines (TCM), possesses anticancer properties. However, the underlying mechanisms of its action remain unclear.  METHODS: Initially, through the application of network pharmacology and molecular docking methodologies, we elucidated the putative core targets and signaling pathways underlying the antineoplastic potential of amentoflavone. Subsequently, we corroborated the therapeutic efficacy and underlying mechanisms of amentoflavone within both in vitro pancreatic cancer cell models and in vivo animal models.<h4>Results</h4>The key targets of amentoflavone we2025-01-01T00:00:00Z2025 Oct2026-06-04T11:45:36.389Z2026-05-08T03:10:51.978ZS-EPMC125216734108592710.1007/s12672-025-03697-4