{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["16(1)"],"submitter":["Shang M"],"pubmed_abstract":["47% of hereditable diseases are caused by single C•G-to-T•A base conversions, which means efficient A-to-G base editing tools (ABEs) have great potential for the treatment of these diseases. However, the existing efficient ABEs, while catalyzing targeted A-to-G conversion, cause high A or C bystander editing and off-target events, which poses safety concerns for their clinical applications. To overcome this shortcoming, we have developed ABE8e-YA (ABE8e with TadA-8e A48E) for efficient and accurate editing of As in YA motifs with YAY > YAR (Y = T or C, R = A or G) hierarchy through structure-oriented rational design. Compared with ABE3.1, which is currently the only ABE version with a YAC motif preference, ABE8e-YA exhibits an average A-to-G editing efficiency improvement of an up to 3.1-f"],"journal":["Nature communications"],"pagination":["9153"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12528748"],"repository":["biostudies-literature"],"pubmed_title":["A motif preferred adenine base editor with minimal bystander and off-targets editing."],"pmcid":["PMC12528748"],"pubmed_authors":["Gonzalez RVL","Li Y","Shang M","Ren J","Zhang X","Zeng Y","Wang J","Cao Q"],"additional_accession":[]},"is_claimable":false,"name":"A motif preferred adenine base editor with minimal bystander and off-targets editing.","description":"47% of hereditable diseases are caused by single C•G-to-T•A base conversions, which means efficient A-to-G base editing tools (ABEs) have great potential for the treatment of these diseases. However, the existing efficient ABEs, while catalyzing targeted A-to-G conversion, cause high A or C bystander editing and off-target events, which poses safety concerns for their clinical applications. To overcome this shortcoming, we have developed ABE8e-YA (ABE8e with TadA-8e A48E) for efficient and accurate editing of As in YA motifs with YAY > YAR (Y = T or C, R = A or G) hierarchy through structure-oriented rational design. Compared with ABE3.1, which is currently the only ABE version with a YAC motif preference, ABE8e-YA exhibits an average A-to-G editing efficiency improvement of an up to 3.1-f","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-07-15T14:33:21.956Z","creation":"2026-07-06T03:08:33.539Z"},"accession":"S-EPMC12528748","cross_references":{"pubmed":["41093849"],"doi":["10.1038/s41467-025-64203-6"]}}