biostudies-literatureUnknown17(5)Jeong JJSeoul National UniversityDiarrhea, a common gastrointestinal symptom in health problems, is highly associated with gut dysbiosis. The purpose of this study is to demonstrate the effect of multistrain probiotics (Sensi-Biome) on diarrhea from the perspective of the microbiome-neuron axis. Sensi-Biome (Lactiplantibacillus plantarum, Bifidobacterium animalis subsp. lactis, Lactobacillus acidophilus, Streptococcus thermophilus, Bifidobacterium bifidum, and Lactococcus lactis) was administered in a 4% acetic acid-induced diarrhea rat model at concentrations of 1 × 10⁸ (G1), 1 × 10⁹ (G2), and 1 × 10¹⁰ CFU/0.5 mL (G3). Diarrhea-related parameters, inflammation-related cytokines, and stool microbiota analysis by 16S rRNA were evaluated. A targeted and untargeted metabolomics approach was uProbiotics and antimicrobial proteins2894-2908https://www.ebi.ac.uk/biostudies/studies/S-EPMC12532644biostudies-literatureMultistrain Probiotics Alleviate Diarrhea by Modulating Microbiome-Derived Metabolites and Serotonin Pathway.PMC12532644Jeong JJYoon SJJin YJJeong MKChoi MRLim JHLee DYSuk KTHan SHPark HJMin UMin BHChoi JJang YJGanesan RSharma SPNa KMHam YLKim BYfalseMultistrain Probiotics Alleviate Diarrhea by Modulating Microbiome-Derived Metabolites and Serotonin Pathway.Diarrhea, a common gastrointestinal symptom in health problems, is highly associated with gut dysbiosis. The purpose of this study is to demonstrate the effect of multistrain probiotics (Sensi-Biome) on diarrhea from the perspective of the microbiome-neuron axis. Sensi-Biome (Lactiplantibacillus plantarum, Bifidobacterium animalis subsp. lactis, Lactobacillus acidophilus, Streptococcus thermophilus, Bifidobacterium bifidum, and Lactococcus lactis) was administered in a 4% acetic acid-induced diarrhea rat model at concentrations of 1 × 10⁸ (G1), 1 × 10⁹ (G2), and 1 × 10¹⁰ CFU/0.5 mL (G3). Diarrhea-related parameters, inflammation-related cytokines, and stool microbiota analysis by 16S rRNA were evaluated. A targeted and untargeted metabolomics approach was u2025-01-01T00:00:00Z2025 Oct2026-06-04T14:34:56.172Z2026-05-10T03:11:20.755ZS-EPMC125326443846792510.1007/s12602-024-10232-4