biostudies-literatureUnknown22(10)Gutierrez-Gonzalez JAAn infusion of Ageratina grandifolia, a traditional preparation, and major compounds [3,5-diprenyl-4-hydroxyacetophenone (1), O-methylencecalinol (2), encecalin (3), and encecalinol (4)], tested at 10, 31.6, and 100 mg/kg, intragastrically administered, showed a significant antinociceptive effect in both phases of the formalin test in mice. Encecalinol (4) was the most active and showed a dose-dependent antinociceptive action in both phases; its effect was substantially attenuated when the mice were pretreated with naloxone. The volatile constituents of A. grandifolia, A. petiolaris, and A. espinosarum were established through gas chromatography-mass spectrometry analysis of the components obtained by head-space solid microextraction and hydrodistillation. The volatiles contained a high peChemistry & biodiversitye00287https://www.ebi.ac.uk/biostudies/studies/S-EPMC12533799biostudies-literatureVolatilome and Antinociceptive Activity of the Traditional Preparation and Compounds from Ageratina grandifolia (Regel) R.M. King & H. Rob.PMC12533799Martinez ALMata RGarcia-Marin GRivero-Cruz IVillasenor JLGutierrez-Gonzalez JAfalseVolatilome and Antinociceptive Activity of the Traditional Preparation and Compounds from Ageratina grandifolia (Regel) R.M. King & H. Rob.An infusion of Ageratina grandifolia, a traditional preparation, and major compounds [3,5-diprenyl-4-hydroxyacetophenone (1), O-methylencecalinol (2), encecalin (3), and encecalinol (4)], tested at 10, 31.6, and 100 mg/kg, intragastrically administered, showed a significant antinociceptive effect in both phases of the formalin test in mice. Encecalinol (4) was the most active and showed a dose-dependent antinociceptive action in both phases; its effect was substantially attenuated when the mice were pretreated with naloxone. The volatile constituents of A. grandifolia, A. petiolaris, and A. espinosarum were established through gas chromatography-mass spectrometry analysis of the components obtained by head-space solid microextraction and hydrodistillation. The volatiles contained a high pe2025-01-01T00:00:00Z2025 Oct2026-06-04T14:32:25.83Z2026-05-10T03:11:11.375ZS-EPMC125337994046877010.1002/cbdv.202500287