biostudies-literatureLi Sthe doctoral research initiation fund projectthe basic scientific research projects of provincial higher education institutions in Heilongjiang Province36410https://www.ebi.ac.uk/biostudies/studies/S-EPMC12534479biostudies-literatureUnknown15(1)Due to cervical cancer poor prognosis, discovering prognostic biomarkers is urgently needed. Numerous publications indicated the presence of stable microRNAs (miRNAs) in human serum. This study aimed to identify a specific panel of serum miRNAs that could be used as reliable biomarkers for cervical cancer prognosis evaluation. The candidate miRNAs were measured using quantitative reverse transcription polymerase chain reaction in serum and tissue samples from patients with either favorable (n = 62) or unfavorable (n = 65) prognosis. These miRNAs were correlated with clinical parameters and patients' survival. Functional assays assessed the role of candidate miRNAs in cervical cancer cells. Receiver operating characteristic curves assessed the candidate miRNAs prediction value for cervical Scientific reportsmiR-378, miR-20a, and miR-520a-3p can be used in a novel serum prognostic panel for cervical cancer.PMC12534479Contract grant number: 2023-KYYWF-0927Contract grant number: 2024-HQBS-01Li HLi YLi SCui YfalsemiR-378, miR-20a, and miR-520a-3p can be used in a novel serum prognostic panel for cervical cancer.Due to cervical cancer poor prognosis, discovering prognostic biomarkers is urgently needed. Numerous publications indicated the presence of stable microRNAs (miRNAs) in human serum. This study aimed to identify a specific panel of serum miRNAs that could be used as reliable biomarkers for cervical cancer prognosis evaluation. The candidate miRNAs were measured using quantitative reverse transcription polymerase chain reaction in serum and tissue samples from patients with either favorable (n = 62) or unfavorable (n = 65) prognosis. These miRNAs were correlated with clinical parameters and patients' survival. Functional assays assessed the role of candidate miRNAs in cervical cancer cells. Receiver operating characteristic curves assessed the candidate miRNAs prediction value for cervical 2025-01-01T00:00:00Z2025 Oct2026-06-04T14:34:10.71Z2026-05-10T03:11:27.93ZS-EPMC125344794110738310.1038/s41598-025-20338-6