biostudies-literatureUnknown16Dias AVL<h4>Introduction</h4>This study aimed to evaluate the effects of sources of non-protein nitrogen (NPN) or rumen undegradable protein (RUP) as supplements on intake, nutrient digestibility, fermentation parameters, and ruminal microbiota in Nellore steers grazing during the dry season.<h4>Methods</h4>The experiment was conducted during the dry season from September to October 2018, in a grazing area of <i>Urochloa brizantha (A. Rich.) Stapf.</i> cv. <i>Xaraés.</i> Eight rumen-and duodenum-cannulated Nellore steers were used in a 4 × 4 Latin square design (2 treatments and 2 periods) balanced for residual effects. The treatments included (1) mineral salt with urea (SMU), formulated to meet 50% of the steer RDP requirement as NPN; and (2) supplementation with corn gluten meal (GLU; 0.3% of body weight) as a source of RUP, with added mineral salt.<h4>Results</h4>GLU supplementation significantly increased supplement intake (<i>p</i> < 0.05) and tended to decrease the intake of forage NDF. Although GLU supplementation improved NDF digestibility, no significant differences were observed in the digestibility of DM, OM, or CP between the treatments (<i>p</i> > 0.05). GLU supplementation significantly increased the relative abundance of several genera, including <i>Ruminococcus 1</i>, <i>Ruminococcus 2</i>, <i>Erysipelotrichaceae UCG-004</i>, <i>Thermoplasmatales Incertae Sedis</i>, <i>Lachnospiraceae XPB1014</i>, <i>Anaeroplasma</i> spp., <i>Anaerotruncus</i> spp. and <i>Eubacterium ruminantium</i> (<i>p</i> < 0.05). The PCA biplot revealed positive associations between GLU supplementation and greater ruminal abundance of <i>Prevotellaceae UCG-004</i> and Bacteroidetes, as well as with higher concentrations of butyrate, propionate, and valerate acids.<h4>Discussion</h4>Supplementation with GLU as a source of RUP in grazing steers during the dry season modulated the rumen microbiota by increasing the abundance of key fibrolytic bacteria and improved fiber digestibility.Frontiers in microbiology1670636https://www.ebi.ac.uk/biostudies/studies/S-EPMC12536655biostudies-literatureAssessing the impact of non-protein nitrogen or rumen undegradable protein supplementation on rumen bacterial diversity and ruminal fermentation in grazing steers during the dry season.PMC12536655Berchielli TTMessana JDDias AVLGranja-Salcedo YTReis RAMachado EVAConstancio MTLCamargo KDVAlves KLGCfalseAssessing the impact of non-protein nitrogen or rumen undegradable protein supplementation on rumen bacterial diversity and ruminal fermentation in grazing steers during the dry season.<h4>Introduction</h4>This study aimed to evaluate the effects of sources of non-protein nitrogen (NPN) or rumen undegradable protein (RUP) as supplements on intake, nutrient digestibility, fermentation parameters, and ruminal microbiota in Nellore steers grazing during the dry season.<h4>Methods</h4>The experiment was conducted during the dry season from September to October 2018, in a grazing area of <i>Urochloa brizantha (A. Rich.) Stapf.</i> cv. <i>Xaraés.</i> Eight rumen-and duodenum-cannulated Nellore steers were used in a 4 × 4 Latin square design (2 treatments and 2 periods) balanced for residual effects. The treatments included (1) mineral salt with urea (SMU), formulated to meet 50% of the steer RDP requirement as NPN; and (2) supplementation with corn gluten meal (GLU; 0.3% of body weight) as a source of RUP, with added mineral salt.<h4>Results</h4>GLU supplementation significantly increased supplement intake (<i>p</i> < 0.05) and tended to decrease the intake of forage NDF. Although GLU supplementation improved NDF digestibility, no significant differences were observed in the digestibility of DM, OM, or CP between the treatments (<i>p</i> > 0.05). GLU supplementation significantly increased the relative abundance of several genera, including <i>Ruminococcus 1</i>, <i>Ruminococcus 2</i>, <i>Erysipelotrichaceae UCG-004</i>, <i>Thermoplasmatales Incertae Sedis</i>, <i>Lachnospiraceae XPB1014</i>, <i>Anaeroplasma</i> spp., <i>Anaerotruncus</i> spp. and <i>Eubacterium ruminantium</i> (<i>p</i> < 0.05). The PCA biplot revealed positive associations between GLU supplementation and greater ruminal abundance of <i>Prevotellaceae UCG-004</i> and Bacteroidetes, as well as with higher concentrations of butyrate, propionate, and valerate acids.<h4>Discussion</h4>Supplementation with GLU as a source of RUP in grazing steers during the dry season modulated the rumen microbiota by increasing the abundance of key fibrolytic bacteria and improved fiber digestibility.2025-01-01T00:00:00Z20252026-05-10T03:25:29.054Z2026-05-10T03:11:41.305ZS-EPMC125366554112245910.3389/fmicb.2025.1670636