{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Torres R"],"funding":["EU","CSIC","FEDER","Agencia Estatal de Investigación","Ministerio de Ciencia e Innovación"],"pagination":["gkaf1051"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12539628"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["53(19)"],"pubmed_abstract":["Natural chromosomal transformation (NCT) in Bacillus subtilis requires RecA and its accessory proteins including RecX and RecD2. Inactivation of the nucleoid-associated protein (NAP) Hbsu, Rok, or LrpC results in a dual effect: it enhances NCT but exacerbates the NCT defect of ΔrecX cells. Purified EbfC exhibits characteristic features of NAPs: it binds both single- and double-stranded DNA, protects them from degradation, and forms higher-order protein-DNA complexes via its DNA bridging activity. NCT is reduced upon EbfC inactivation but enhanced by ebfC overexpression. hbs55, Δrok, or ΔlrpC mutations suppress the NCT defect of ΔebfC, yet synergistically increase NCT upon ebfC overexpression. The NCT defect in ΔrecD2 cells is worsened by ebfC overexpression or by hbs55, Δrok, or ΔlrpC muta"],"journal":["Nucleic acids research"],"pubmed_title":["Nucleoid structure and dynamics influence natural chromosomal transformation in Bacillus subtilis: the role of EbfC."],"pmcid":["PMC12539628"],"funding_grant_id":["202520E100","PID2021-122273NB-I00"],"pubmed_authors":["Morikawa K","Alonso JC","Lopez-Sanz M","Ushijima Y","Torres R"],"additional_accession":[]},"is_claimable":false,"name":"Nucleoid structure and dynamics influence natural chromosomal transformation in Bacillus subtilis: the role of EbfC.","description":"Natural chromosomal transformation (NCT) in Bacillus subtilis requires RecA and its accessory proteins including RecX and RecD2. Inactivation of the nucleoid-associated protein (NAP) Hbsu, Rok, or LrpC results in a dual effect: it enhances NCT but exacerbates the NCT defect of ΔrecX cells. Purified EbfC exhibits characteristic features of NAPs: it binds both single- and double-stranded DNA, protects them from degradation, and forms higher-order protein-DNA complexes via its DNA bridging activity. NCT is reduced upon EbfC inactivation but enhanced by ebfC overexpression. hbs55, Δrok, or ΔlrpC mutations suppress the NCT defect of ΔebfC, yet synergistically increase NCT upon ebfC overexpression. The NCT defect in ΔrecD2 cells is worsened by ebfC overexpression or by hbs55, Δrok, or ΔlrpC muta","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-06-04T15:22:24.343Z","creation":"2026-05-10T03:12:26.567Z"},"accession":"S-EPMC12539628","cross_references":{"pubmed":["41118573"],"doi":["10.1093/nar/gkaf1051"]}}