biostudies-literatureChen Wthe Foundation of Guangdong Basic and Applied Basic Research Foundation1926https://www.ebi.ac.uk/biostudies/studies/S-EPMC12540211biostudies-literatureUnknown16(1)<h4>Background</h4>Osteosarcoma is an aggressive bone malignancy with high metastatic potential and poor prognosis, primarily affecting children and adolescents. Although lysyl oxidase-like 4 (LOXL4) has been implicated in tumor progression, its functional role and mechanistic contributions in Osteosarcoma remain unclear.<h4>Methods</h4>We performed integrated bioinformatics analysis using GTEx, TARGET, and TCGA datasets to evaluate LOXL4 expression and prognostic significance across cancers. Genetic alteration, immune infiltration, and RNA methylation analysis were carried to explore the different roles of LOXL4 in tumors. Functional assays, including Cell Counting Kit-8 (CCK-8), colony formation, and Matrigel transwell assays, were conducted in Osteosarcoma cell lines. Besides, western bDiscover oncologyPan-cancer analysis of role of LOXL4 and experiment validation in osteosarcoma.PMC125402112023A1515140045 & 2023A1515140129 & 2023A1515140183 & 2023A1515140034Lv JHuang SXie FHuang DWen ZZhong RChen WZheng SZhong HSun JLiu WfalsePan-cancer analysis of role of LOXL4 and experiment validation in osteosarcoma.<h4>Background</h4>Osteosarcoma is an aggressive bone malignancy with high metastatic potential and poor prognosis, primarily affecting children and adolescents. Although lysyl oxidase-like 4 (LOXL4) has been implicated in tumor progression, its functional role and mechanistic contributions in Osteosarcoma remain unclear.<h4>Methods</h4>We performed integrated bioinformatics analysis using GTEx, TARGET, and TCGA datasets to evaluate LOXL4 expression and prognostic significance across cancers. Genetic alteration, immune infiltration, and RNA methylation analysis were carried to explore the different roles of LOXL4 in tumors. Functional assays, including Cell Counting Kit-8 (CCK-8), colony formation, and Matrigel transwell assays, were conducted in Osteosarcoma cell lines. Besides, western b2025-01-01T00:00:00Z2025 Oct2026-06-10T05:18:28.646Z2026-06-10T03:07:43.349ZS-EPMC125402114111801210.1007/s12672-025-03761-z