biostudies-literatureRaduwan HNational Institute of Allergy and Infectious Diseases Division of Intramural ResearchHoward Hughes Medical InstituteNIAID NIH HHSNational Institutes of HealthNIH HHS113666https://www.ebi.ac.uk/biostudies/studies/S-EPMC12554125biostudies-literatureUnknown28(11)The influence of the time of day on the cutaneous immune response to mosquito feeding is not well understood. <i>Aedes aegypti</i> mosquitoes feed on mice throughout the day, and a bloodmeal is most often obtained at times of day that are equivalent to dawn (ZT1) and dusk (ZT11). We observed that cells in the murine skin elicited more differentially expressed genes at ZT11 compared to ZT1. Additionally, we detected more immune cells in the skin at ZT11 in response to a mosquito bite. These results suggest that assessments of host responses to a mosquito bite and mosquito-borne infections may be influenced by the diurnal rhythm.iScienceThe cutaneous response to a mosquito bite is influenced by the diurnal rhythm.PMC12554125S10 OD030363AI142624R01 AI1426241S10OD030363-01A1Marin-Lopez ARaduwan HFikrig EPark JMontgomery RRFlavell RASkadow MHChen TYShaw ACfalseThe cutaneous response to a mosquito bite is influenced by the diurnal rhythm.The influence of the time of day on the cutaneous immune response to mosquito feeding is not well understood. <i>Aedes aegypti</i> mosquitoes feed on mice throughout the day, and a bloodmeal is most often obtained at times of day that are equivalent to dawn (ZT1) and dusk (ZT11). We observed that cells in the murine skin elicited more differentially expressed genes at ZT11 compared to ZT1. Additionally, we detected more immune cells in the skin at ZT11 in response to a mosquito bite. These results suggest that assessments of host responses to a mosquito bite and mosquito-borne infections may be influenced by the diurnal rhythm.2025-01-01T00:00:00Z2025 Nov2026-06-10T05:21:54.242Z2026-06-10T03:07:39.232ZS-EPMC125541254114670710.1016/j.isci.2025.113666