{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang Z"],"funding":["Tsinghua Initiative Scientific Research Program","National Natural Science Foundation of China","Beijing Natural Science Foundation"],"pagination":["113687"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12554141"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["28(11)"],"pubmed_abstract":["Microglia represent critical therapeutic targets in spinal cord injury (SCI), with damage-associated microglia (DAM) playing key roles in neuroinflammation and tissue repair. Through integrated in-silico analysis of single-cell RNA sequencing (scRNA-seq) and microarray datasets, we identified DAM subsets specific to acute SCI characterized by hub genes <i>Fcer1g</i>, <i>Grn</i>, and <i>Gusb</i>. Using a C57BL/6 mouse spinal cord contusion model, we validated increased DAM accumulation post-injury and demonstrated their propensity to transition toward homeostatic microglia (MG2). Eupatilin treatment promoted DAM-to-MG2 differentiation, as confirmed through bulk and scRNA-seq analyses, revealing supportive gene expression changes. These findings establish DAM as functionally distinct microglial populations in acute SCI and identify Eupatilin as a therapeutic agent that facilitates beneficial microglial polarization. This work provides mechanistic insights into microglial dynamics during SCI and suggests targeted modulation of DAM-to-MG2 transitions as a promising therapeutic strategy for promoting inflammation resolution and functional recovery."],"journal":["iScience"],"pubmed_title":["Eupatilin ameliorates spinal cord injury by inhibiting damage-associated microglia and optimizing the regenerative microenvironment."],"pmcid":["PMC12554141"],"funding_grant_id":["20257020014","82301560","7242187","82201521"],"pubmed_authors":["Liu Y","Yang J","Su B","Luan G","Yu B","Yang K","Wang G","Wang X","Man W","Meng Z","Wang Z","Zhang P"],"additional_accession":[]},"is_claimable":false,"name":"Eupatilin ameliorates spinal cord injury by inhibiting damage-associated microglia and optimizing the regenerative microenvironment.","description":"Microglia represent critical therapeutic targets in spinal cord injury (SCI), with damage-associated microglia (DAM) playing key roles in neuroinflammation and tissue repair. Through integrated in-silico analysis of single-cell RNA sequencing (scRNA-seq) and microarray datasets, we identified DAM subsets specific to acute SCI characterized by hub genes <i>Fcer1g</i>, <i>Grn</i>, and <i>Gusb</i>. Using a C57BL/6 mouse spinal cord contusion model, we validated increased DAM accumulation post-injury and demonstrated their propensity to transition toward homeostatic microglia (MG2). Eupatilin treatment promoted DAM-to-MG2 differentiation, as confirmed through bulk and scRNA-seq analyses, revealing supportive gene expression changes. These findings establish DAM as functionally distinct microglial populations in acute SCI and identify Eupatilin as a therapeutic agent that facilitates beneficial microglial polarization. This work provides mechanistic insights into microglial dynamics during SCI and suggests targeted modulation of DAM-to-MG2 transitions as a promising therapeutic strategy for promoting inflammation resolution and functional recovery.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-05T05:17:55.529Z","creation":"2026-06-04T03:06:09.095Z"},"accession":"S-EPMC12554141","cross_references":{"pubmed":["41146716"],"doi":["10.1016/j.isci.2025.113687"]}}