biostudies-literatureWolfgramm HDeutsche Forschungsgemeinschaft100489https://www.ebi.ac.uk/biostudies/studies/S-EPMC12554148biostudies-literatureUnknown9Mouse-adapted <i>Staphylococcus aureus</i> strains have become increasingly relevant in infection research thanks to their ability to better recapitulate clinical infection dynamics in mouse models. However, detailed characterisations required to establish a corresponding reference strain are still lacking. The mouse-adapted CC88 strain JSNZ appears to be an ideal candidate for a reference strain. Here, we present a comprehensive genomic and proteomic characterisation of JSNZ. Whole genome sequencing was performed using a combination of short and long reads. Proteomic profiling was conducted under standard laboratory conditions in TSB and RPMI during exponential and stationary growth using LC-MS/MS. The updated, closed genome sequence of JSNZ was integrated into <i>Aureo</i>Wiki for user-fCurrent research in microbial sciencesIntegrated genomic and proteomic analysis of the mouse-adapted &lt;i&gt;Staphylococcus aureus&lt;/i&gt; strain JSNZ.PMC12554148443535983Holtfreter STebben JGesell Salazar MSteil LMader USurmann KSura TVolker UMichalik SMehlan HBludau EReder AWolfgramm HBusch LMHoffmuller THagenau LfalseIntegrated genomic and proteomic analysis of the mouse-adapted &lt;i&gt;Staphylococcus aureus&lt;/i&gt; strain JSNZ.Mouse-adapted <i>Staphylococcus aureus</i> strains have become increasingly relevant in infection research thanks to their ability to better recapitulate clinical infection dynamics in mouse models. However, detailed characterisations required to establish a corresponding reference strain are still lacking. The mouse-adapted CC88 strain JSNZ appears to be an ideal candidate for a reference strain. Here, we present a comprehensive genomic and proteomic characterisation of JSNZ. Whole genome sequencing was performed using a combination of short and long reads. Proteomic profiling was conducted under standard laboratory conditions in TSB and RPMI during exponential and stationary growth using LC-MS/MS. The updated, closed genome sequence of JSNZ was integrated into <i>Aureo</i>Wiki for user-f2025-01-01T00:00:00Z20252026-07-16T15:12:47.514Z2026-07-09T11:04:36.798ZS-EPMC125541484114672510.1016/j.crmicr.2025.100489