biostudies-literatureUnknown10(1)Dennis NMeasurement of the oxygen consumption rate, or respirometry, is a powerful and comprehensive method for assessing mitochondrial function both in vitro and in vivo. Respirometry at the whole-organism level has been repeatedly performed in the model organism Caenorhabditis elegans, typically using high-throughput microplate-based systems over traditional Clark-type respirometers. However, these systems are highly specialized, costly to purchase and operate, and inaccessible to many researchers. Here, we develop a respirometry assay using low-cost commercially available optical oxygen sensors (PreSens OxoPlates®) and fluorescence plate readers (the BMG FLUOstar), as an alternative to more costly standard respirometry systems. This assay uses standard BMG FLUOstBiology methods & protocolsbpaf072https://www.ebi.ac.uk/biostudies/studies/S-EPMC12557035biostudies-literatureA novel C. elegans respirometry assay using low-cost optical oxygen sensors.PMC12557035Dennis NGourlay CWEzcurra MfalseA novel C. elegans respirometry assay using low-cost optical oxygen sensors.Measurement of the oxygen consumption rate, or respirometry, is a powerful and comprehensive method for assessing mitochondrial function both in vitro and in vivo. Respirometry at the whole-organism level has been repeatedly performed in the model organism Caenorhabditis elegans, typically using high-throughput microplate-based systems over traditional Clark-type respirometers. However, these systems are highly specialized, costly to purchase and operate, and inaccessible to many researchers. Here, we develop a respirometry assay using low-cost commercially available optical oxygen sensors (PreSens OxoPlates®) and fluorescence plate readers (the BMG FLUOstar), as an alternative to more costly standard respirometry systems. This assay uses standard BMG FLUOst2025-01-01T00:00:00Z20252026-05-14T03:19:56.553Z2026-05-14T03:12:35.015ZS-EPMC125570354115894910.1093/biomethods/bpaf072