biostudies-literatureUnknown16Kara E<h4>Introduction</h4><i>In vitro</i> and <i>in vivo</i> in rodent models were used to study the effects of a human follicle-stimulating hormone (hFSH)-potentiating monoclonal antibody (IGX12) on hFSH bioactivity.<h4>Methods</h4>Potentiation of recombinant hFSH (rhFSH)-, human luteinizing hormone/chorionic gonadotropin (hLH/hCG)-, or human thyroid stimulating hormone (TSH)-mediated cyclic adenosine monophosphate (cAMP) induction <i>in vitro</i> was performed using HEK 293 cells overexpressing either human FSH receptor (hFSH-R), human LH-receptor (hLH-R) or human thyroid stimulating hormone receptor (hTSH-R). The effect of rhFSH on ovarian weight and hCG on seminal vesicle weight was tested in immature female or male rats. Potentiation of spermatogenesis was examined in adult male rats with Frontiers in endocrinology1668945https://www.ebi.ac.uk/biostudies/studies/S-EPMC12558798biostudies-literatureFirst in class monoclonal antibody potentiating human follicle stimulating hormone activity improves spermatogenesis in azoospermic rodent models.PMC12558798Frydman RBouchard PMaurel MCKara ECasteret SDecourtye JDupuy LfalseFirst in class monoclonal antibody potentiating human follicle stimulating hormone activity improves spermatogenesis in azoospermic rodent models.<h4>Introduction</h4><i>In vitro</i> and <i>in vivo</i> in rodent models were used to study the effects of a human follicle-stimulating hormone (hFSH)-potentiating monoclonal antibody (IGX12) on hFSH bioactivity.<h4>Methods</h4>Potentiation of recombinant hFSH (rhFSH)-, human luteinizing hormone/chorionic gonadotropin (hLH/hCG)-, or human thyroid stimulating hormone (TSH)-mediated cyclic adenosine monophosphate (cAMP) induction <i>in vitro</i> was performed using HEK 293 cells overexpressing either human FSH receptor (hFSH-R), human LH-receptor (hLH-R) or human thyroid stimulating hormone receptor (hTSH-R). The effect of rhFSH on ovarian weight and hCG on seminal vesicle weight was tested in immature female or male rats. Potentiation of spermatogenesis was examined in adult male rats with 2025-01-01T00:00:00Z20252026-06-05T09:10:06.053Z2026-05-15T03:12:32.14ZS-EPMC125587984116368210.3389/fendo.2025.1668945