biostudies-literatureUnknown88(10)Lopez-Sampedro ESea cucumbers (Holothuriidae) are highly valued in traditional medicine and are recognized for their diverse bioactivities, which are largely attributed to their rich triterpene glycoside content. Given that <i>Holothuria inornata</i> was chemically uncharacterized, this study describes the bioassay-guided isolation of these compounds. Nine sulfated saponins were purified, and their structures were elucidated using UPLC-QTOF/HR-ESI-MS<sup><i>n</i></sup> and comprehensive NMR spectroscopy. Six new compounds were identified, including inornatosides A-C (<b>1</b>-<b>3</b>) and 22<i>R</i>-holothurin B (<b>6</b>), all possessing a holostane-type aglycone, alongside inornatosides D (<b>4</b>) and E (<b>5</b>), which feature unique aglycones. Cytotoxic assays revealed that inornatoside B (<b>2</bJournal of natural products2372-2382https://www.ebi.ac.uk/biostudies/studies/S-EPMC12560079biostudies-literatureCytotoxic Triterpene Glycosides from Mexican Sea Cucumber <i>Holothuria inornata</i>.PMC12560079Lopez-Sampedro EArreguin-Espinosa RSimonet AMfalseCytotoxic Triterpene Glycosides from Mexican Sea Cucumber <i>Holothuria inornata</i>.Sea cucumbers (Holothuriidae) are highly valued in traditional medicine and are recognized for their diverse bioactivities, which are largely attributed to their rich triterpene glycoside content. Given that <i>Holothuria inornata</i> was chemically uncharacterized, this study describes the bioassay-guided isolation of these compounds. Nine sulfated saponins were purified, and their structures were elucidated using UPLC-QTOF/HR-ESI-MS<sup><i>n</i></sup> and comprehensive NMR spectroscopy. Six new compounds were identified, including inornatosides A-C (<b>1</b>-<b>3</b>) and 22<i>R</i>-holothurin B (<b>6</b>), all possessing a holostane-type aglycone, alongside inornatosides D (<b>4</b>) and E (<b>5</b>), which feature unique aglycones. Cytotoxic assays revealed that inornatoside B (<b>2</b2025-01-01T00:00:00Z2025 Oct2026-06-05T08:49:48.391Z2026-05-14T03:12:33.772ZS-EPMC125600794097694810.1021/acs.jnatprod.5c00716