biostudies-literatureYusuph QNational Institute of General Medical SciencesNIGMS NIH HHS20809-20816https://www.ebi.ac.uk/biostudies/studies/S-EPMC12560149biostudies-literatureUnknown97(38)Carbohydrates are found in various forms in living organisms, both as free-standing glycans as well as glycoconjugates including glycoproteins, glycolipids, and glycosaminoglycans. These structures play crucial roles in many biological processes, often mediated or influenced by interactions of carbohydrates with other biomolecules. However, studying these interactions is particularly challenging due to the structural complexity of carbohydrates, their dynamic conformational behavior, and the low binding affinities often involved. To address these challenges, we are developing a novel method that leverages mass spectrometry-based radical carbohydrate footprinting (RCF). We monitored changes in the solvent accessibility of specific regions within oligosaccharides by measuring variations in tAnalytical chemistryHydroxyl and Trifluoromethyl Radical Carbohydrate Footprinting for Probing Protein Binding Components of Oligosaccharides.PMC12560149R01 GM127267R01GM127267P20GM130460P20 GM130460Liu HYusuph QSharp JSMisra SKfalseHydroxyl and Trifluoromethyl Radical Carbohydrate Footprinting for Probing Protein Binding Components of Oligosaccharides.Carbohydrates are found in various forms in living organisms, both as free-standing glycans as well as glycoconjugates including glycoproteins, glycolipids, and glycosaminoglycans. These structures play crucial roles in many biological processes, often mediated or influenced by interactions of carbohydrates with other biomolecules. However, studying these interactions is particularly challenging due to the structural complexity of carbohydrates, their dynamic conformational behavior, and the low binding affinities often involved. To address these challenges, we are developing a novel method that leverages mass spectrometry-based radical carbohydrate footprinting (RCF). We monitored changes in the solvent accessibility of specific regions within oligosaccharides by measuring variations in t2025-01-01T00:00:00Z2025 Sep2026-06-05T09:29:41.808Z2026-05-15T03:12:09.232ZS-EPMC125601494097302810.1021/acs.analchem.5c02719