biostudies-literatureUnknown14(10)Piatti G<b>Background</b>: Molecular techniques for microbial identification have highlighted the relevance of polymicrobial infections in humans, such as those affecting the urinary tract. Although in vitro investigations have demonstrated connections between co-infections and microbial interaction, their role is unclear in clinics, given the overlap with host conditions. <b>Objective</b>: We aimed to separate the roles of organisms and patient conditions in human polymicrobial urinary samples by performing a relevant epidemiological analysis. <b>Methods</b>: We analyzed retrospective results from urine cultures performed during one year in a 1200 beds Italian hospital. Patients were grouped as uncompromised and compromised and positive urine cultures were grouped as monomicrobial and polymicrobiAntibiotics (Basel, Switzerland)1028https://www.ebi.ac.uk/biostudies/studies/S-EPMC12561107biostudies-literatureEpidemiological Evidence Supports the Role of Microbial Interactions in Polymicrobial UTI Infections Revealed by In Vitro Research.PMC12561107Piatti GMannini AVitale ABruzzone MCeppi MSchito AMfalseEpidemiological Evidence Supports the Role of Microbial Interactions in Polymicrobial UTI Infections Revealed by In Vitro Research.<b>Background</b>: Molecular techniques for microbial identification have highlighted the relevance of polymicrobial infections in humans, such as those affecting the urinary tract. Although in vitro investigations have demonstrated connections between co-infections and microbial interaction, their role is unclear in clinics, given the overlap with host conditions. <b>Objective</b>: We aimed to separate the roles of organisms and patient conditions in human polymicrobial urinary samples by performing a relevant epidemiological analysis. <b>Methods</b>: We analyzed retrospective results from urine cultures performed during one year in a 1200 beds Italian hospital. Patients were grouped as uncompromised and compromised and positive urine cultures were grouped as monomicrobial and polymicrobi2025-01-01T00:00:00Z2025 Oct2026-05-14T03:14:50.931Z2026-05-14T03:09:09.794ZS-EPMC125611074114872010.3390/antibiotics14101028