biostudies-literatureUnknown14(10)Giannotti L<h4>Background</h4>Glioblastoma (GBM) is a highly aggressive tumor characterized by elevated plasticity and poor differentiation. Platelet-derived preparations such as Concentrated Growth Factors (CGF) are rich in bioactive molecules, but their effects on tumor biology remain underexplored.<h4>Methods</h4>U87MG glioblastoma cells were cultured with a conditioned medium obtained from CGF over 14 days (CGF-CM). We analyzed cell viability, morphology, DNA integrity, migration, proliferation, and expression of astrocytic markers.<h4>Results</h4>CGF-CM treatment induced early enhancement of cell viability, followed by decreased proliferation and reduced migration at later time points. Morphological analyses revealed astrocyte-like features. The expression of glial fibrillary acidic protein (GFABiology1461https://www.ebi.ac.uk/biostudies/studies/S-EPMC12561328biostudies-literatureCGF-Conditioned Medium Modulates Astrocytic Differentiation and Invasiveness in U87MG Glioblastoma Cells.PMC12561328Demitri CDamiano FPalermo ASpedicato FDe Sangro MAStanca EFerrante FSiculella LGiannotti LDi Chiara Stanca BAbbruzzese LfalseCGF-Conditioned Medium Modulates Astrocytic Differentiation and Invasiveness in U87MG Glioblastoma Cells.<h4>Background</h4>Glioblastoma (GBM) is a highly aggressive tumor characterized by elevated plasticity and poor differentiation. Platelet-derived preparations such as Concentrated Growth Factors (CGF) are rich in bioactive molecules, but their effects on tumor biology remain underexplored.<h4>Methods</h4>U87MG glioblastoma cells were cultured with a conditioned medium obtained from CGF over 14 days (CGF-CM). We analyzed cell viability, morphology, DNA integrity, migration, proliferation, and expression of astrocytic markers.<h4>Results</h4>CGF-CM treatment induced early enhancement of cell viability, followed by decreased proliferation and reduced migration at later time points. Morphological analyses revealed astrocyte-like features. The expression of glial fibrillary acidic protein (GFA2025-01-01T00:00:00Z2025 Oct2026-05-15T03:10:50.383Z2026-05-15T03:07:50.424ZS-EPMC125613284115486310.3390/biology14101461