biostudies-literatureLi CDeutsche ForschungsgemeinschaftEuropean Research CouncilNIDDK NIH HHSChinese Scholarship Council. Deutsche ForschungsgemeinschaftProf. Martha Merrow and Dr. Francesca Sartor of the LMU generously provided access to and expertise regarding the Nikon Eclipse Ti2 microscope systemNational Institutes of HealthNIH HHSe06707https://www.ebi.ac.uk/biostudies/studies/S-EPMC12561471biostudies-literatureUnknown12(40)RNA-binding proteins regulate post-transcriptional gene translation, but the macrophage-specific role of Zc3h12c remains poorly characterized. Here, the role of Zc3h12c in macrophages is characterized using Tnfrsf11a^Cre-Zc3h12c^flox/flox mice. Both Tnfrsf11a and Zc3h12c are highly expressed in the kidney tissue from patients with chronic kidney disease and showed a positive association with an interstitial fibrosis score. Single cell RNA sequencing demonstrated abundant Tnfrsf11a expression in murine kidney macrophages and a correlation with the induction of chemokines, macrophage phagocytosis, and activation upon kidney injury. In various kidney injury models, Tnfrsf11a^Cre-Zc3h12c^flox/flox mice suffered from more injury and inflammation in the kiAdvanced science (Weinheim, Baden-Wurttemberg, Germany)Macrophage Zc3h12c Limits Tissue Inflammation and Injury via Alternative Splicing of Pre-mRNA.PMC12561471R01DK076077INST 86/1851-1 FUGGR01 DK087635SFB TRR332R01 DK076077R01 DK105821R01DK105821R01DK087635648274GZ:INST 86/1851-1 FUGGAN372/30-1TRR332Li CZhu ZLech MSusztak KSchulz CZhang NKusunoki YMarschner JAWatanabe-Kusunoki KZhao ZDeng HLi XAnders HJSteiger SXu YfalseMacrophage Zc3h12c Limits Tissue Inflammation and Injury via Alternative Splicing of Pre-mRNA.RNA-binding proteins regulate post-transcriptional gene translation, but the macrophage-specific role of Zc3h12c remains poorly characterized. Here, the role of Zc3h12c in macrophages is characterized using Tnfrsf11a^Cre-Zc3h12c^flox/flox mice. Both Tnfrsf11a and Zc3h12c are highly expressed in the kidney tissue from patients with chronic kidney disease and showed a positive association with an interstitial fibrosis score. Single cell RNA sequencing demonstrated abundant Tnfrsf11a expression in murine kidney macrophages and a correlation with the induction of chemokines, macrophage phagocytosis, and activation upon kidney injury. In various kidney injury models, Tnfrsf11a^Cre-Zc3h12c^flox/flox mice suffered from more injury and inflammation in the ki2025-01-01T00:00:00Z2025 Oct2026-06-05T08:58:16.455Z2026-05-15T03:07:54.636ZS-EPMC125614714083442910.1002/advs.202506707