{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang X"],"funding":["Shandong Provincial Natural Science Foundation"],"pagination":["3049"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12562201"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(20)"],"pubmed_abstract":["Heat stress (HS) severely significantly reduces milk yield and causes substantial economic losses of dairy cows. TMT-based proteomes and an untargeted metabolomics approach were used to conduct the proteomics and metabolomics in heat-stressed (HS, <i>n</i> = 6) and heat-resistant (HR, <i>n</i> = 6) Chinese Holstein. The proteomics showed that 29 differentially expressed proteins (DEPs), with SERPINA3-7, ACTN4, and PLOD1 up-regulated, and GSN down-regulated in HR cows. The metabolomics showed that 168 differential positive metabolites and 170 differential negative metabolites were identified, with HR cows exhibiting lower levels of anti-inflammatory compounds, such as N6-Acetyl-L-lysine. In addition, 29 DEPs and 338 metabolites revealed four key pathways, including the lysine degradation (k"],"journal":["Animals : an open access journal from MDPI"],"pubmed_title":["Integrated Analysis of Proteomics and Metabolomics for Heat Stress in Chinese Holstein Cows."],"pmcid":["PMC12562201"],"funding_grant_id":["ZR2023QC252"],"pubmed_authors":["Liu H","Yang J","Li J","Wang C","Gao H","Li R","Pei F","Bao P","Zhao X","Hou M","Gao Y","Wang X","Hao D","Yuan Y"],"additional_accession":[]},"is_claimable":false,"name":"Integrated Analysis of Proteomics and Metabolomics for Heat Stress in Chinese Holstein Cows.","description":"Heat stress (HS) severely significantly reduces milk yield and causes substantial economic losses of dairy cows. TMT-based proteomes and an untargeted metabolomics approach were used to conduct the proteomics and metabolomics in heat-stressed (HS, <i>n</i> = 6) and heat-resistant (HR, <i>n</i> = 6) Chinese Holstein. The proteomics showed that 29 differentially expressed proteins (DEPs), with SERPINA3-7, ACTN4, and PLOD1 up-regulated, and GSN down-regulated in HR cows. The metabolomics showed that 168 differential positive metabolites and 170 differential negative metabolites were identified, with HR cows exhibiting lower levels of anti-inflammatory compounds, such as N6-Acetyl-L-lysine. In addition, 29 DEPs and 338 metabolites revealed four key pathways, including the lysine degradation (k","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-05-15T03:19:49.456Z","creation":"2026-05-15T03:12:41.874Z"},"accession":"S-EPMC12562201","cross_references":{"pubmed":["41153976"],"doi":["10.3390/ani15203049"]}}