biostudies-literatureBraga KMSRhode Island FoundationAmerican Heart Association Visiting Professorship AwardNHLBI NIH HHSCNPq (National Council for Scientific and Technological Development- Brazil)NIGMS NIH HHSCOBRE Stem Cells and Aging of National Institute of Health1167https://www.ebi.ac.uk/biostudies/studies/S-EPMC12562227biostudies-literatureUnknown14(10)Reactive oxygen species (ROS) overproduction contributes to endothelial dysfunction in Type 2 diabetes mellitus (T2DM). Pequi (<i>Caryocar brasiliense</i>, Camb), a native Brazilian fruit, is rich in polyphenolic antioxidants. We investigated whether its ethanolic bark extract modulates ROS levels and promotes proliferation in human coronary artery endothelial cells from patients with diabetes (D-HCAECs). Cells were treated with pequi extract under normoxic, hypoxic, or H<sub>2</sub>O<sub>2</sub>-induced oxidative stress conditions. Cytosolic and mitochondrial ROS levels, cell proliferation, and the expression of antioxidant proteins (Nrf2, HO-1, SOD1, SOD2, catalase, and GPx1) were assessed. Pequi significantly reduced cytosolic ROS under normoxia and both cytosolic and mitochondrial ROS Antioxidants (Basel, Switzerland)Pequi (<i>Caryocar brasiliense</i>, Camb) Bark Extract Reduces ROS Production in Diabetic Human Coronary Endothelial Cells.PMC12562227P30 GM145500R01 HL175045R56 HL133624208126/2017-1R56HL1336245P30GM14550025IVPHA1460933R01 HL046716R01 HL1288311472420231352Sellke FWAraujo EGBraga KMSAbid MRfalsePequi (<i>Caryocar brasiliense</i>, Camb) Bark Extract Reduces ROS Production in Diabetic Human Coronary Endothelial Cells.Reactive oxygen species (ROS) overproduction contributes to endothelial dysfunction in Type 2 diabetes mellitus (T2DM). Pequi (<i>Caryocar brasiliense</i>, Camb), a native Brazilian fruit, is rich in polyphenolic antioxidants. We investigated whether its ethanolic bark extract modulates ROS levels and promotes proliferation in human coronary artery endothelial cells from patients with diabetes (D-HCAECs). Cells were treated with pequi extract under normoxic, hypoxic, or H<sub>2</sub>O<sub>2</sub>-induced oxidative stress conditions. Cytosolic and mitochondrial ROS levels, cell proliferation, and the expression of antioxidant proteins (Nrf2, HO-1, SOD1, SOD2, catalase, and GPx1) were assessed. Pequi significantly reduced cytosolic ROS under normoxia and both cytosolic and mitochondrial ROS 2025-01-01T00:00:00Z2025 Sep2026-05-15T03:21:28.375Z2026-05-15T03:12:43.349ZS-EPMC125622274115447610.3390/antiox14101167