{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bork I"],"funding":["Deutsche Forschungsgemeinschaft"],"pagination":["643"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12562644"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(10)"],"pubmed_abstract":["Lactate is a key metabolite with applications ranging from monitoring training efficiency to early sepsis detection and monitoring the metabolic state of cell cultures. In this study, a paper-based lactate sensor utilizing a fluorescent readout was developed. Unlike common lactate dehydrogenase (LDH)-based methods, these sensors use a green fluorescent protein (GFP) or mApple-coupled lactate binding domain, which provides a fluorescent readout upon lactate binding. We demonstrate that immobilizing these proteins on paper does not affect their ability to bind lactate and produce a fluorescent readout, by monitoring lactate levels in the cell culture supernatant applying different cell culture conditions."],"journal":["Biosensors"],"pubmed_title":["Covalent and Site-Specific Immobilization of a Fluorogenic Sensor Protein on Cellulose-Based Paper for Detection of Lactate in Cell Culture Media."],"pmcid":["PMC12562644"],"funding_grant_id":["DFG Grant KO 1390/16-1"],"pubmed_authors":["Becker J","Biesalski M","Hofling V","Meckel T","Kolmar H","Bork I"],"additional_accession":[]},"is_claimable":false,"name":"Covalent and Site-Specific Immobilization of a Fluorogenic Sensor Protein on Cellulose-Based Paper for Detection of Lactate in Cell Culture Media.","description":"Lactate is a key metabolite with applications ranging from monitoring training efficiency to early sepsis detection and monitoring the metabolic state of cell cultures. In this study, a paper-based lactate sensor utilizing a fluorescent readout was developed. Unlike common lactate dehydrogenase (LDH)-based methods, these sensors use a green fluorescent protein (GFP) or mApple-coupled lactate binding domain, which provides a fluorescent readout upon lactate binding. We demonstrate that immobilizing these proteins on paper does not affect their ability to bind lactate and produce a fluorescent readout, by monitoring lactate levels in the cell culture supernatant applying different cell culture conditions.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-05-14T03:12:16.986Z","creation":"2026-05-14T03:08:49.573Z"},"accession":"S-EPMC12562644","cross_references":{"pubmed":["41149296"],"doi":["10.3390/bios15100643"]}}