{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhu J"],"funding":["Major Science and Technology Projects in Sichuan Province","Sichuan Science and Technology Program"],"pagination":["3530"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12563075"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(20)"],"pubmed_abstract":["Waxy rice starch (WRS), characterized by low amylose content, high viscosity, and strong gel-forming ability, is highly valued in food and industrial applications. Temperature-sensitive genic male-sterile (TGMS) lines exhibit complete male sterility under low-temperature conditions, a trait widely exploited in hybrid rice breeding. Here, we generated an elite waxy TGMS line, 520S, via CRISPR/Cas9-mediated editing of the Waxy (<i>Wx</i>) gene. The <i>wx</i> mutants displayed robust male sterility, desirable glutinous traits, and favorable physicochemical properties, including gelatinization temperature, gel consistency, paste viscosity, and amylopectin fine structure. Fertility assays confirmed temperature-sensitive pollen sterility consistent with wild-type responses, and T2 generation mut"],"journal":["Foods (Basel, Switzerland)"],"pubmed_title":["Creation of TGMS Lines of Waxy Rice with Elite Physicochemical Properties of Starch via Waxy Gene Editing."],"pmcid":["PMC12563075"],"funding_grant_id":["2022ZDZX0016","2023JDRC0133"],"pubmed_authors":["Hua Y","Fu Y","Li P","Xu H","Li W","Liu Y","Wei M","Zhu J","Li S","Zhao R","Wan J","Sun H","Wang Z","Gan T","Luo T"],"additional_accession":[]},"is_claimable":false,"name":"Creation of TGMS Lines of Waxy Rice with Elite Physicochemical Properties of Starch via Waxy Gene Editing.","description":"Waxy rice starch (WRS), characterized by low amylose content, high viscosity, and strong gel-forming ability, is highly valued in food and industrial applications. Temperature-sensitive genic male-sterile (TGMS) lines exhibit complete male sterility under low-temperature conditions, a trait widely exploited in hybrid rice breeding. Here, we generated an elite waxy TGMS line, 520S, via CRISPR/Cas9-mediated editing of the Waxy (<i>Wx</i>) gene. The <i>wx</i> mutants displayed robust male sterility, desirable glutinous traits, and favorable physicochemical properties, including gelatinization temperature, gel consistency, paste viscosity, and amylopectin fine structure. Fertility assays confirmed temperature-sensitive pollen sterility consistent with wild-type responses, and T2 generation mut","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-06-04T03:12:59.903Z","creation":"2026-06-04T03:06:20.018Z"},"accession":"S-EPMC12563075","cross_references":{"pubmed":["41154066"],"doi":["10.3390/foods14203530"]}}