biostudies-literatureZhu JMajor Science and Technology Projects in Sichuan ProvinceSichuan Science and Technology Program3530https://www.ebi.ac.uk/biostudies/studies/S-EPMC12563075biostudies-literatureUnknown14(20)Waxy rice starch (WRS), characterized by low amylose content, high viscosity, and strong gel-forming ability, is highly valued in food and industrial applications. Temperature-sensitive genic male-sterile (TGMS) lines exhibit complete male sterility under low-temperature conditions, a trait widely exploited in hybrid rice breeding. Here, we generated an elite waxy TGMS line, 520S, via CRISPR/Cas9-mediated editing of the Waxy (<i>Wx</i>) gene. The <i>wx</i> mutants displayed robust male sterility, desirable glutinous traits, and favorable physicochemical properties, including gelatinization temperature, gel consistency, paste viscosity, and amylopectin fine structure. Fertility assays confirmed temperature-sensitive pollen sterility consistent with wild-type responses, and T2 generation mutFoods (Basel, Switzerland)Creation of TGMS Lines of Waxy Rice with Elite Physicochemical Properties of Starch via Waxy Gene Editing.PMC125630752022ZDZX00162023JDRC0133Hua YFu YLi PXu HLi WLiu YWei MZhu JLi SZhao RWan JSun HWang ZGan TLuo TfalseCreation of TGMS Lines of Waxy Rice with Elite Physicochemical Properties of Starch via Waxy Gene Editing.Waxy rice starch (WRS), characterized by low amylose content, high viscosity, and strong gel-forming ability, is highly valued in food and industrial applications. Temperature-sensitive genic male-sterile (TGMS) lines exhibit complete male sterility under low-temperature conditions, a trait widely exploited in hybrid rice breeding. Here, we generated an elite waxy TGMS line, 520S, via CRISPR/Cas9-mediated editing of the Waxy (<i>Wx</i>) gene. The <i>wx</i> mutants displayed robust male sterility, desirable glutinous traits, and favorable physicochemical properties, including gelatinization temperature, gel consistency, paste viscosity, and amylopectin fine structure. Fertility assays confirmed temperature-sensitive pollen sterility consistent with wild-type responses, and T2 generation mut2025-01-01T00:00:00Z2025 Oct2026-06-04T03:12:59.903Z2026-06-04T03:06:20.018ZS-EPMC125630754115406610.3390/foods14203530