biostudies-literatureTurcov DMinistry of Education and Research1371https://www.ebi.ac.uk/biostudies/studies/S-EPMC12563663biostudies-literatureUnknown15(10)The growing need for environmentally friendly separation processes has motivated the search for alternative solvents to petroleum-derived chemicals for the recovery of biosynthesized products. Although effective, conventional petroleum-based solvents pose major environmental and sustainability concerns, including pollution, ecotoxicity, human health risks, and high costs and energy demands for recycling. Consequently, current research and industrial practice increasingly focus on their replacement with safer and more sustainable alternatives. This study investigates the use of natural oils (i.e., grapeseed, sweet almond, and flaxseed oils) as renewable, biodegradable, and non-toxic diluents in reactive extraction systems for the separation of 7-aminocephalosporanic acid (7-ACA). The combinBiomoleculesNatural Oils as Green Solvents for Reactive Extraction of 7-Aminocephalosporanic Acid: A Sustainable Approach to Bioproduct Recovery in Environmental Biotechnology.PMC12563663CNFIS-FDI-2025-F-0177Tucaliuc ATurcov DGalaction AICascaval DParaschiv MBlaga ACfalseNatural Oils as Green Solvents for Reactive Extraction of 7-Aminocephalosporanic Acid: A Sustainable Approach to Bioproduct Recovery in Environmental Biotechnology.The growing need for environmentally friendly separation processes has motivated the search for alternative solvents to petroleum-derived chemicals for the recovery of biosynthesized products. Although effective, conventional petroleum-based solvents pose major environmental and sustainability concerns, including pollution, ecotoxicity, human health risks, and high costs and energy demands for recycling. Consequently, current research and industrial practice increasingly focus on their replacement with safer and more sustainable alternatives. This study investigates the use of natural oils (i.e., grapeseed, sweet almond, and flaxseed oils) as renewable, biodegradable, and non-toxic diluents in reactive extraction systems for the separation of 7-aminocephalosporanic acid (7-ACA). The combin2025-01-01T00:00:00Z2025 Sep2026-05-14T03:19:43.96Z2026-05-14T03:12:28.456ZS-EPMC125636634115460110.3390/biom15101371