biostudies-literatureTelli AESelcuk University Scientific Research and Project Coordination3573https://www.ebi.ac.uk/biostudies/studies/S-EPMC12564342biostudies-literatureUnknown14(20)<b>Background:</b> The emergence of extended-spectrum β-lactamase (ESBL) producing and colistin-resistant <i>Escherichia coli</i> in retail meat poses a significant public health risk. <b>Method:</b> A total of 180 retail meat samples (chicken parts, internals, processed products; lamb; beef; fish) were purchased from markets and butcher shops across Turkiye. Presumptive ESBL-producing isolates were screened on chromogenic agar and phenotypically confirmed. Species identity was verified by <i>uspA</i> PCR, and resistance genes (<i>blaCTX-M</i>, <i>blaTEM</i>, <i>blaOXA</i>, <i>blaSHV</i>, <i>mcr</i>-1, <i>mcr</i>-2, <i>mcr</i>-3) were analyzed. Colistin MICs were determined by broth microdilution, while antimicrobial susceptibility of ESBL-positive isolates was assessed by disk diffusion. Foods (Basel, Switzerland)Co-Occurrence and Molecular Characterization of ESBL-Producing and Colistin-Resistant <i>Escherichia coli</i> Isolates from Retail Raw Meat.PMC1256434221401116Bicer YYılmaz TTelli AETurkal GUcar GTelli NfalseCo-Occurrence and Molecular Characterization of ESBL-Producing and Colistin-Resistant <i>Escherichia coli</i> Isolates from Retail Raw Meat.<b>Background:</b> The emergence of extended-spectrum β-lactamase (ESBL) producing and colistin-resistant <i>Escherichia coli</i> in retail meat poses a significant public health risk. <b>Method:</b> A total of 180 retail meat samples (chicken parts, internals, processed products; lamb; beef; fish) were purchased from markets and butcher shops across Turkiye. Presumptive ESBL-producing isolates were screened on chromogenic agar and phenotypically confirmed. Species identity was verified by <i>uspA</i> PCR, and resistance genes (<i>blaCTX-M</i>, <i>blaTEM</i>, <i>blaOXA</i>, <i>blaSHV</i>, <i>mcr</i>-1, <i>mcr</i>-2, <i>mcr</i>-3) were analyzed. Colistin MICs were determined by broth microdilution, while antimicrobial susceptibility of ESBL-positive isolates was assessed by disk diffusion. 2025-01-01T00:00:00Z2025 Oct2026-05-14T03:13:55.946Z2026-05-14T03:08:57.734ZS-EPMC125643424115410910.3390/foods14203573