biostudies-literatureMessias TSCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - Brasil2388https://www.ebi.ac.uk/biostudies/studies/S-EPMC12566026biostudies-literatureUnknown13(10)Filoviruses are among the most lethal viral human pathogens known, with significant relevance to public health, yet their evolutionary history remains poorly resolved. This study applied a multiprotein molecular phylogenetic approach to investigate the evolutionary and temporal dynamics of the family <i>Filoviridae</i>. Amino acid sequences from the proteome and seven individual proteins (NP, VP35, VP40, GP, VP30, VP24, L) were analyzed using MEGA 12, with RelTime inference anchored on uniform calibrations, and integration of epidemiological data (cases, fatalities, case fatality). The phylogenetic reconstructions revealed robust topologies for most proteins, though selective pressures on GP, VP30 and VP40 generated more variable patterns. Temporal inferences supported the classification oMicroorganismsExploratory Temporal and Evolutionary Insights into the &lt;i&gt;Filoviridae&lt;/i&gt; Family Through Multiprotein Phylogeny.PMC12566026grant#305801/2024-4Vieira NAVieira ISStefani MJCQuerino GAMengoa MGRIbanes ASSilva KCPArantes SCMarcos ECOlivo TETOliveira TMMelo ECLuz PCRDBattochio APRMessias TSSoares SfalseExploratory Temporal and Evolutionary Insights into the &lt;i&gt;Filoviridae&lt;/i&gt; Family Through Multiprotein Phylogeny.Filoviruses are among the most lethal viral human pathogens known, with significant relevance to public health, yet their evolutionary history remains poorly resolved. This study applied a multiprotein molecular phylogenetic approach to investigate the evolutionary and temporal dynamics of the family <i>Filoviridae</i>. Amino acid sequences from the proteome and seven individual proteins (NP, VP35, VP40, GP, VP30, VP24, L) were analyzed using MEGA 12, with RelTime inference anchored on uniform calibrations, and integration of epidemiological data (cases, fatalities, case fatality). The phylogenetic reconstructions revealed robust topologies for most proteins, though selective pressures on GP, VP30 and VP40 generated more variable patterns. Temporal inferences supported the classification o2025-01-01T00:00:00Z2025 Oct2026-05-15T03:10:27.163Z2026-05-15T03:07:48.293ZS-EPMC125660264115684710.3390/microorganisms13102388