biostudies-literatureCai Zthe Qinghai Provincial Natural Science FoundationQinghai Provincial Science and Technology Commissioner Program: Demonstration and Promotion of Organic Forage Cultivation Technologies in Dari County2341https://www.ebi.ac.uk/biostudies/studies/S-EPMC12566440biostudies-literatureUnknown13(10)The restoration of "Heitutan" degraded grasslands on the Qinghai-Tibetan Plateau was hindered by suboptimal grass species mixtures, leading to low vegetation productivity, impaired soil nutrient cycling, and microbial functional degradation. Based on a 22-year controlled field experiment, this study systematically elucidated the regulatory mechanisms of different artificial grass mixtures on vegetation community characteristics, soil physicochemical properties, and bacterial community structure and function. The results demonstrated that mixed-sowing treatments significantly improved soil conditions and enhanced aboveground biomass. The HC treatment (<i>Elymus nutans</i> Griseb. + <i>Poa crymophila</i> Keng ex L. Liu cv. 'Qinghai' + <i>Festuca sinensis</i> Keng ex S. L. Lu cv. 'Qinghai') aMicroorganismsEcological Effects and Microbial Regulatory Mechanisms of Functional Grass Species Assembly in the Restoration of "Heitutan" Degraded Alpine Grasslands.PMC125664402025-NK-P252023-ZJ-923MLv LFu SLi FZhang HCai ZLiu QShi JBao SfalseEcological Effects and Microbial Regulatory Mechanisms of Functional Grass Species Assembly in the Restoration of "Heitutan" Degraded Alpine Grasslands.The restoration of "Heitutan" degraded grasslands on the Qinghai-Tibetan Plateau was hindered by suboptimal grass species mixtures, leading to low vegetation productivity, impaired soil nutrient cycling, and microbial functional degradation. Based on a 22-year controlled field experiment, this study systematically elucidated the regulatory mechanisms of different artificial grass mixtures on vegetation community characteristics, soil physicochemical properties, and bacterial community structure and function. The results demonstrated that mixed-sowing treatments significantly improved soil conditions and enhanced aboveground biomass. The HC treatment (<i>Elymus nutans</i> Griseb. + <i>Poa crymophila</i> Keng ex L. Liu cv. 'Qinghai' + <i>Festuca sinensis</i> Keng ex S. L. Lu cv. 'Qinghai') a2025-01-01T00:00:00Z2025 Oct2026-05-14T03:17:34.127Z2026-05-14T03:12:01.449ZS-EPMC125664404115679910.3390/microorganisms13102341