{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yun H"],"funding":["South Korean Government"],"pagination":["2320"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12566482"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(10)"],"pubmed_abstract":["Microbial forensics involves analyzing biological evidence to evaluate weaponized microorganisms or their toxins. This study aimed to detect and type <i>Yersinia pestis</i> from four simulated forensic samples-human plasma diluted in phosphate-buffered saline (#24-2), tomato juice (#24-5), grape juice (#24-8), and a surgical mask (#24-10). Notably, samples #24-10 may have contained live bacteria other than <i>Y. pestis</i>. A real-time polymerase chain reaction confirmed the presence of <i>Y. pestis</i> in all samples; however, whole-genome sequencing (WGS) coverage of the <i>Y. pestis</i> chromosome ranged from 0.46% to 97.1%, largely due to host DNA interference and low abundance. To address these limitations and enable strain-level identification, we designed a hybridization-based targe"],"journal":["Microorganisms"],"pubmed_title":["Typing of &lt;i&gt;Yersinia pestis&lt;/i&gt; in Challenging Forensic Samples Through Targeted Next-Generation Sequencing of Multilocus Variable Number Tandem Repeat Regions."],"pmcid":["PMC12566482"],"funding_grant_id":["912A01201"],"pubmed_authors":["Song DH","Yun H","Lee SH","Gu SH","Lim SH"],"additional_accession":[]},"is_claimable":false,"name":"Typing of &lt;i&gt;Yersinia pestis&lt;/i&gt; in Challenging Forensic Samples Through Targeted Next-Generation Sequencing of Multilocus Variable Number Tandem Repeat Regions.","description":"Microbial forensics involves analyzing biological evidence to evaluate weaponized microorganisms or their toxins. This study aimed to detect and type <i>Yersinia pestis</i> from four simulated forensic samples-human plasma diluted in phosphate-buffered saline (#24-2), tomato juice (#24-5), grape juice (#24-8), and a surgical mask (#24-10). Notably, samples #24-10 may have contained live bacteria other than <i>Y. pestis</i>. A real-time polymerase chain reaction confirmed the presence of <i>Y. pestis</i> in all samples; however, whole-genome sequencing (WGS) coverage of the <i>Y. pestis</i> chromosome ranged from 0.46% to 97.1%, largely due to host DNA interference and low abundance. To address these limitations and enable strain-level identification, we designed a hybridization-based targe","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-05-15T03:16:33.533Z","creation":"2026-05-15T03:11:49.556Z"},"accession":"S-EPMC12566482","cross_references":{"pubmed":["41156780"],"doi":["10.3390/microorganisms13102320"]}}