{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Simioni B"],"funding":["Fundação Araucária","Superintendência Geral de Ciência, Tecnologia e Ensino Superior do Estado do Paraná","CNPq"],"pagination":["2367"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12566495"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(10)"],"pubmed_abstract":["The present study investigated the impact of genetic engineering strategies to produce a cell-free xylanase for applications in the baking industry. The <i>xynA1</i> gene from the nonpathogenic bacterium <i>Caulobacter vibrioides</i> was integrated into the pAS22 vector with a xylose-inducible promoter and introduced back into the bacteria, resulting in the creation of the BS-<i>xynA1</i>. This construct exhibited substantial secreted xylanase 1 (XynA1) activity, reaching 17.22 U/mL, and a specific activity of 278.64 U/mg after an 18 h growth period with 0.3% (<i>v</i>/<i>v</i>) xylose plus 0.2% (<i>w</i>/<i>v</i>) corn straw. RT-qPCR analysis confirmed that higher xylanase activity in <i>C. vibrioides</i> cells was correlated with increased transcription of the <i>xynA1</i> gene in the in"],"journal":["Microorganisms"],"pubmed_title":["Bioengineering &lt;i&gt;Caulobacter vibrioides&lt;/i&gt; for Xylanase Applications in the Bakery Industry."],"pmcid":["PMC12566495"],"funding_grant_id":["Emenda Governamental-EG n◦ 14/2024-Termo de Execução Descentralizada -TED n◦ 032/2025","Project nº: PRD2023361000397 Agreement nº 180/2025 PDI","Universal Grant: 407596/2023-2"],"pubmed_authors":["Rocha PMC","Maller A","Gandra RF","Kadowaki MK","Simioni B","Favero A","da Conceicao Silva JL","Simao RCG"],"additional_accession":[]},"is_claimable":false,"name":"Bioengineering &lt;i&gt;Caulobacter vibrioides&lt;/i&gt; for Xylanase Applications in the Bakery Industry.","description":"The present study investigated the impact of genetic engineering strategies to produce a cell-free xylanase for applications in the baking industry. The <i>xynA1</i> gene from the nonpathogenic bacterium <i>Caulobacter vibrioides</i> was integrated into the pAS22 vector with a xylose-inducible promoter and introduced back into the bacteria, resulting in the creation of the BS-<i>xynA1</i>. This construct exhibited substantial secreted xylanase 1 (XynA1) activity, reaching 17.22 U/mL, and a specific activity of 278.64 U/mg after an 18 h growth period with 0.3% (<i>v</i>/<i>v</i>) xylose plus 0.2% (<i>w</i>/<i>v</i>) corn straw. RT-qPCR analysis confirmed that higher xylanase activity in <i>C. vibrioides</i> cells was correlated with increased transcription of the <i>xynA1</i> gene in the in","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-05-14T03:21:31.87Z","creation":"2026-05-14T03:12:49.253Z"},"accession":"S-EPMC12566495","cross_references":{"pubmed":["41156826"],"doi":["10.3390/microorganisms13102367"]}}