biostudies-literatureSimioni BFundação AraucáriaSuperintendência Geral de Ciência, Tecnologia e Ensino Superior do Estado do ParanáCNPq2367https://www.ebi.ac.uk/biostudies/studies/S-EPMC12566495biostudies-literatureUnknown13(10)The present study investigated the impact of genetic engineering strategies to produce a cell-free xylanase for applications in the baking industry. The <i>xynA1</i> gene from the nonpathogenic bacterium <i>Caulobacter vibrioides</i> was integrated into the pAS22 vector with a xylose-inducible promoter and introduced back into the bacteria, resulting in the creation of the BS-<i>xynA1</i>. This construct exhibited substantial secreted xylanase 1 (XynA1) activity, reaching 17.22 U/mL, and a specific activity of 278.64 U/mg after an 18 h growth period with 0.3% (<i>v</i>/<i>v</i>) xylose plus 0.2% (<i>w</i>/<i>v</i>) corn straw. RT-qPCR analysis confirmed that higher xylanase activity in <i>C. vibrioides</i> cells was correlated with increased transcription of the <i>xynA1</i> gene in the inMicroorganismsBioengineering <i>Caulobacter vibrioides</i> for Xylanase Applications in the Bakery Industry.PMC12566495Emenda Governamental-EG n◦ 14/2024-Termo de Execução Descentralizada -TED n◦ 032/2025Project nº: PRD2023361000397 Agreement nº 180/2025 PDIUniversal Grant: 407596/2023-2Rocha PMCMaller AGandra RFKadowaki MKSimioni BFavero Ada Conceicao Silva JLSimao RCGfalseBioengineering <i>Caulobacter vibrioides</i> for Xylanase Applications in the Bakery Industry.The present study investigated the impact of genetic engineering strategies to produce a cell-free xylanase for applications in the baking industry. The <i>xynA1</i> gene from the nonpathogenic bacterium <i>Caulobacter vibrioides</i> was integrated into the pAS22 vector with a xylose-inducible promoter and introduced back into the bacteria, resulting in the creation of the BS-<i>xynA1</i>. This construct exhibited substantial secreted xylanase 1 (XynA1) activity, reaching 17.22 U/mL, and a specific activity of 278.64 U/mg after an 18 h growth period with 0.3% (<i>v</i>/<i>v</i>) xylose plus 0.2% (<i>w</i>/<i>v</i>) corn straw. RT-qPCR analysis confirmed that higher xylanase activity in <i>C. vibrioides</i> cells was correlated with increased transcription of the <i>xynA1</i> gene in the in2025-01-01T00:00:00Z2025 Oct2026-05-14T03:21:31.87Z2026-05-14T03:12:49.253ZS-EPMC125664954115682610.3390/microorganisms13102367