biostudies-literatureHong Lthe Science and Technology Development Fund, Macau SARthe University of MacauZhuhai Science and Technology Plan Project in the Social Development Field1444https://www.ebi.ac.uk/biostudies/studies/S-EPMC12566646biostudies-literatureUnknown18(10)<b>Background/Objectives:</b> Allergic diseases (e.g., asthma, chronic urticaria) are increasing globally, but current anti-allergic drugs exhibit limitations in efficacy and safety. Traditional Chinese Medicine (TCM) emphasizes constitutional regulation for allergic diseases management. The allergic constitution prescription (ACP), a TCM formulation, lacks clear mechanistic insights. <b>Methods:</b> This study employs a novel network pharmacology approach integrating ultra-high performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) to identify ACP's chemical components and compare its mechanisms with anti-allergic drugs. Chemical components of ACP were analyzed via UHPLC-Q-TOF-MS/MS, and allergic disease-related targets were collected fromPharmaceuticals (Basel, Switzerland)Characterization and Anti-Allergic Mechanisms of Bioactive Compounds in a Traditional Chinese Medicine Prescription Using UHPLC-Q-TOF-MS/MS, Network Pharmacology and Computational Simulations.PMC125666460005/2024/AKP2220004000117MYRG-GRG2024-00150-ICMS-UMDFCPG2025-00030-ICMSHong LWang QLi SIp CQin YZeng HXu WWang JZhao JDuan XfalseCharacterization and Anti-Allergic Mechanisms of Bioactive Compounds in a Traditional Chinese Medicine Prescription Using UHPLC-Q-TOF-MS/MS, Network Pharmacology and Computational Simulations.<b>Background/Objectives:</b> Allergic diseases (e.g., asthma, chronic urticaria) are increasing globally, but current anti-allergic drugs exhibit limitations in efficacy and safety. Traditional Chinese Medicine (TCM) emphasizes constitutional regulation for allergic diseases management. The allergic constitution prescription (ACP), a TCM formulation, lacks clear mechanistic insights. <b>Methods:</b> This study employs a novel network pharmacology approach integrating ultra-high performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UHPLC-Q-TOF-MS/MS) to identify ACP's chemical components and compare its mechanisms with anti-allergic drugs. Chemical components of ACP were analyzed via UHPLC-Q-TOF-MS/MS, and allergic disease-related targets were collected from2025-01-01T00:00:00Z2025 Sep2026-05-14T03:20:53.813Z2026-05-14T03:12:45.277ZS-EPMC125666464115555910.3390/ph18101444