{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chang HY"],"funding":["Ministry of Higher Education, Malaysia","Universiti Malaya","University of Malaya","Ministry of Higher Education"],"pagination":["1017"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12567581"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(10)"],"pubmed_abstract":["<b>Background/Objectives</b>: Enterovirus A71 (EV-A71) and coxsackievirus A16 (CVA16) are major causative agents of hand, foot and mouth disease (HFMD), often co-circulating and occasionally undergoing genetic recombination. While natural recombinants often involve genomic regions encoding non-structural proteins, their effects on replication and pathogenesis remain unclear. <b>Methods</b>: To address this, four chimera viruses (Chi-CCE, Chi-ECE, Chi-EEC, and Chi-CEC) were constructed with 5'UTR, capsid P1, and non-structural P2 and P3 genes, from CVA16 (denoted as C) or EV-A71 (denoted as E). These chimeras were tested for replication kinetics and cytopathic effects in rhabdomyosarcoma cells while <i>in vivo</i> virulence and protection efficacy were evaluated using a newborn BALB/c mouse"],"journal":["Vaccines"],"pubmed_title":["Intertypic Recombination Between Coxsackievirus A16 and Enterovirus A71 Structural and Non-Structural Genes Modulates Virulence and Protection Efficacy."],"pmcid":["PMC12567581"],"funding_grant_id":["MG001-2024","RK009-2021","FRGS/1/2018/SKK11/UM/02/2","PV001-2019"],"pubmed_authors":["Sam IC","Chang HY","Jasni K","Chan YF","Tee HK","Ong KC","Abdullah S"],"additional_accession":[]},"is_claimable":false,"name":"Intertypic Recombination Between Coxsackievirus A16 and Enterovirus A71 Structural and Non-Structural Genes Modulates Virulence and Protection Efficacy.","description":"<b>Background/Objectives</b>: Enterovirus A71 (EV-A71) and coxsackievirus A16 (CVA16) are major causative agents of hand, foot and mouth disease (HFMD), often co-circulating and occasionally undergoing genetic recombination. While natural recombinants often involve genomic regions encoding non-structural proteins, their effects on replication and pathogenesis remain unclear. <b>Methods</b>: To address this, four chimera viruses (Chi-CCE, Chi-ECE, Chi-EEC, and Chi-CEC) were constructed with 5'UTR, capsid P1, and non-structural P2 and P3 genes, from CVA16 (denoted as C) or EV-A71 (denoted as E). These chimeras were tested for replication kinetics and cytopathic effects in rhabdomyosarcoma cells while <i>in vivo</i> virulence and protection efficacy were evaluated using a newborn BALB/c mouse","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Sep","modification":"2026-07-10T03:26:11.531Z","creation":"2026-07-10T03:16:15.239Z"},"accession":"S-EPMC12567581","cross_references":{"pubmed":["41150405"],"doi":["10.3390/vaccines13101017"]}}