{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kuhn S"],"funding":["Deutsche Forschungsgemeinschaft","Deutsche Forschungsgemeinschaft (German Research Foundation)","École Polytechnique Fédérale de Lausanne","Max-Planck-Gesellschaft"],"pagination":["1754-1761"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12568630"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["21(11)"],"pubmed_abstract":["SNAP-tag is a powerful tool for labeling proteins with synthetic fluorophores in bioimaging. However, its utility in live-cell applications can be constrained by its relatively slow labeling kinetics and the limited cell permeability of its substrates. Here, we introduce improved labeling substrates and an engineered SNAP-tag for faster labeling in vitro and in live cells. SNAP-tag2 presents a second-order rate constant with rhodamine substrates that approaches 10<sup>7</sup> s<sup>-1</sup> M<sup>-1</sup>, a 100-fold improvement over the corresponding SNAP-tag-substrate pairs. When labeled with highly fluorogenic dyes, SNAP-tag2 also shows a fivefold increase in fluorescence brightness relative to currently used SNAP-tag. The increased labeling kinetics and brightness of SNAP-tag2 translat"],"journal":["Nature chemical biology"],"pubmed_title":["SNAP-tag2 for faster and brighter protein labeling."],"pmcid":["PMC12568630"],"funding_grant_id":["TRR 186"],"pubmed_authors":["Johnsson K","de Lange EMF","Egoldt C","Lennoi A","Tarnawski M","Lin YH","Vlijm R","Kompa J","Reinstein J","Nasufovic V","Hiblot J","Wilhelm J","Fischer J","Kuhn S"],"additional_accession":[]},"is_claimable":false,"name":"SNAP-tag2 for faster and brighter protein labeling.","description":"SNAP-tag is a powerful tool for labeling proteins with synthetic fluorophores in bioimaging. However, its utility in live-cell applications can be constrained by its relatively slow labeling kinetics and the limited cell permeability of its substrates. Here, we introduce improved labeling substrates and an engineered SNAP-tag for faster labeling in vitro and in live cells. SNAP-tag2 presents a second-order rate constant with rhodamine substrates that approaches 10<sup>7</sup> s<sup>-1</sup> M<sup>-1</sup>, a 100-fold improvement over the corresponding SNAP-tag-substrate pairs. When labeled with highly fluorogenic dyes, SNAP-tag2 also shows a fivefold increase in fluorescence brightness relative to currently used SNAP-tag. The increased labeling kinetics and brightness of SNAP-tag2 translat","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-05T07:12:01.551Z","creation":"2026-05-14T03:08:46.152Z"},"accession":"S-EPMC12568630","cross_references":{"pubmed":["40610720"],"doi":["10.1038/s41589-025-01942-z"]}}