biostudies-literatureUnknown10(41)Lee YOral delivery of cannabidiol (CBD) is challenging because of its poor water solubility and low bioavailability, which restrict its therapeutic potential. In this study, we used a CBD-loaded oil-in-water nanoemulsion prepared from octenyl succinate anhydride (OSA)-modified starch to address these challenges. The CBD-encapsulated nanoemulsion (CBD-NE) exhibited a uniform particle size of 39.18 ± 0.15 nm, high encapsulation efficiency of 99.80 ± 0.13%, and maintained colloidal stability during storage for 28 days. Simulated gastrointestinal digestion demonstrated that the bioaccessibility of CBD in CBD-NE was higher than that in unformulated CBD. Furthermore, <i>in vitro</i> experiments using RAW264.7 macrophages showed that CBD-NE significantly (<i>p</i> < 0.05) suppressed lipopolysaccharideACS omega48146-48154https://www.ebi.ac.uk/biostudies/studies/S-EPMC12573299biostudies-literatureCannabidiol Loaded Nanoemulsion with Improved Bioaccessibility and Anti-Inflammatory Activity.PMC12573299Song HYKim SHByun EBLee YfalseCannabidiol Loaded Nanoemulsion with Improved Bioaccessibility and Anti-Inflammatory Activity.Oral delivery of cannabidiol (CBD) is challenging because of its poor water solubility and low bioavailability, which restrict its therapeutic potential. In this study, we used a CBD-loaded oil-in-water nanoemulsion prepared from octenyl succinate anhydride (OSA)-modified starch to address these challenges. The CBD-encapsulated nanoemulsion (CBD-NE) exhibited a uniform particle size of 39.18 ± 0.15 nm, high encapsulation efficiency of 99.80 ± 0.13%, and maintained colloidal stability during storage for 28 days. Simulated gastrointestinal digestion demonstrated that the bioaccessibility of CBD in CBD-NE was higher than that in unformulated CBD. Furthermore, <i>in vitro</i> experiments using RAW264.7 macrophages showed that CBD-NE significantly (<i>p</i> < 0.05) suppressed lipopolysaccharide2025-01-01T00:00:00Z2025 Oct2026-06-05T09:43:00.588Z2026-05-15T03:12:57.913ZS-EPMC125732994118007210.1021/acsomega.5c04608