{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kim S"],"funding":["NIBIB NIH HHS","NIAID NIH HHS","National Institutes of Health"],"pagination":["6268-6280"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12574115"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["25(23)"],"pubmed_abstract":["Effective monitoring of HIV treatment remains constrained by limited access to affordable, sensitive, and user-friendly viral load diagnostics, particularly in resource-limited settings. To address this critical gap, a fully automated, point-of-care device was developed that integrates microfluidic sample processing with ultrasensitive bioluminescence-based viral load detection. Upon simple sample loading, the system automates all steps, capturing multiple HIV subtypes with antibody-coated magnetic beads without fold-change, amplifying bioluminescence <i>via</i> enzyme cascades, and quantifying viral load using a low-cost optical sensor. Analytical validation using 87 HIV-spiked plasma samples confirmed a 95 copies per mL detection limit and reliable quantification of viral loads. Clinical"],"journal":["Lab on a chip"],"pubmed_title":["A luminescence-based point-of-care HIV viral load test for antiretroviral therapy monitoring."],"pmcid":["PMC12574115"],"funding_grant_id":["UM1 AI106701","R61AI140489","R01EB033866","R01AI138800","UM1 AI068634","R61 AI140489","UM1AI068636","UM1 AI068636","UM1AI068634","R01 AI138800","R33AI140489","UM1AI106701","R01AI138800-05S1","R01 EB033866","R33 AI140489"],"pubmed_authors":["Hong HR","Oh JM","Gwak J","Lee J","Kim J","Li JZ","Chun J","Hardie JM","Kuritzkes DR","Husain SM","Kim S","Kanakasabapathy MK","Tsibris AM","Chellappaa A","Yoo J","Shafiee H","Thirumalaraju P","Song Y"],"additional_accession":[]},"is_claimable":false,"name":"A luminescence-based point-of-care HIV viral load test for antiretroviral therapy monitoring.","description":"Effective monitoring of HIV treatment remains constrained by limited access to affordable, sensitive, and user-friendly viral load diagnostics, particularly in resource-limited settings. To address this critical gap, a fully automated, point-of-care device was developed that integrates microfluidic sample processing with ultrasensitive bioluminescence-based viral load detection. Upon simple sample loading, the system automates all steps, capturing multiple HIV subtypes with antibody-coated magnetic beads without fold-change, amplifying bioluminescence <i>via</i> enzyme cascades, and quantifying viral load using a low-cost optical sensor. Analytical validation using 87 HIV-spiked plasma samples confirmed a 95 copies per mL detection limit and reliable quantification of viral loads. Clinical","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-06T15:46:14.983Z","creation":"2026-06-02T03:09:23.903Z"},"accession":"S-EPMC12574115","cross_references":{"pubmed":["41114948"],"doi":["10.1039/d5lc00636h"]}}