{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cho Y"],"funding":["National Research Foundation of Korea"],"pagination":["322"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12576987"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["31(1)"],"pubmed_abstract":["<h4>Background</h4>Coactivator-associated arginine methyltransferase 1 (CARM1) regulates diverse cellular processes-including transcription, cell cycle progression, metabolism, and autophagy-through asymmetric dimethylation of both histone and non-histone substrates. Although TP-064 and EZM2302 both inhibit CARM1, they may elicit distinct biological effects.<h4>Methods</h4>We employed immunoblotting, subcellular fractionation, histone extraction, chromatin immunoprecipitation assay, quantitative PCR, and confocal microscopy to compare the effects of TP-064 and EZM2302. Substrate methylation and autophagic responses were evaluated under nutrient-deprived conditions.<h4>Results</h4>Both TP-064 and EZM2302 inhibited CARM1-dependent methylation of non-histone substrates, including p300, GAPDH,"],"journal":["Molecular medicine (Cambridge, Mass.)"],"pubmed_title":["Context-specific applications of CARM1 inhibitors: functional profiles of EZM2302 and TP-064."],"pmcid":["PMC12576987"],"funding_grant_id":["RS-2025-00563180","RS-2024-00412728"],"pubmed_authors":["Cho Y","Kim YK"],"additional_accession":[]},"is_claimable":false,"name":"Context-specific applications of CARM1 inhibitors: functional profiles of EZM2302 and TP-064.","description":"<h4>Background</h4>Coactivator-associated arginine methyltransferase 1 (CARM1) regulates diverse cellular processes-including transcription, cell cycle progression, metabolism, and autophagy-through asymmetric dimethylation of both histone and non-histone substrates. Although TP-064 and EZM2302 both inhibit CARM1, they may elicit distinct biological effects.<h4>Methods</h4>We employed immunoblotting, subcellular fractionation, histone extraction, chromatin immunoprecipitation assay, quantitative PCR, and confocal microscopy to compare the effects of TP-064 and EZM2302. Substrate methylation and autophagic responses were evaluated under nutrient-deprived conditions.<h4>Results</h4>Both TP-064 and EZM2302 inhibited CARM1-dependent methylation of non-histone substrates, including p300, GAPDH,","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-06-05T10:34:12.613Z","creation":"2026-05-16T03:08:03.245Z"},"accession":"S-EPMC12576987","cross_references":{"pubmed":["41174476"],"doi":["10.1186/s10020-025-01388-y"]}}