biostudies-literatureCho YNational Research Foundation of Korea322https://www.ebi.ac.uk/biostudies/studies/S-EPMC12576987biostudies-literatureUnknown31(1)<h4>Background</h4>Coactivator-associated arginine methyltransferase 1 (CARM1) regulates diverse cellular processes-including transcription, cell cycle progression, metabolism, and autophagy-through asymmetric dimethylation of both histone and non-histone substrates. Although TP-064 and EZM2302 both inhibit CARM1, they may elicit distinct biological effects.<h4>Methods</h4>We employed immunoblotting, subcellular fractionation, histone extraction, chromatin immunoprecipitation assay, quantitative PCR, and confocal microscopy to compare the effects of TP-064 and EZM2302. Substrate methylation and autophagic responses were evaluated under nutrient-deprived conditions.<h4>Results</h4>Both TP-064 and EZM2302 inhibited CARM1-dependent methylation of non-histone substrates, including p300, GAPDH,Molecular medicine (Cambridge, Mass.)Context-specific applications of CARM1 inhibitors: functional profiles of EZM2302 and TP-064.PMC12576987RS-2025-00563180RS-2024-00412728Cho YKim YKfalseContext-specific applications of CARM1 inhibitors: functional profiles of EZM2302 and TP-064.<h4>Background</h4>Coactivator-associated arginine methyltransferase 1 (CARM1) regulates diverse cellular processes-including transcription, cell cycle progression, metabolism, and autophagy-through asymmetric dimethylation of both histone and non-histone substrates. Although TP-064 and EZM2302 both inhibit CARM1, they may elicit distinct biological effects.<h4>Methods</h4>We employed immunoblotting, subcellular fractionation, histone extraction, chromatin immunoprecipitation assay, quantitative PCR, and confocal microscopy to compare the effects of TP-064 and EZM2302. Substrate methylation and autophagic responses were evaluated under nutrient-deprived conditions.<h4>Results</h4>Both TP-064 and EZM2302 inhibited CARM1-dependent methylation of non-histone substrates, including p300, GAPDH,2025-01-01T00:00:00Z2025 Oct2026-06-05T10:34:12.613Z2026-05-16T03:08:03.245ZS-EPMC125769874117447610.1186/s10020-025-01388-y