biostudies-literatureUnknown33(4)Suuring MCell therapy is a promising approach for inducing tolerance and thus reducing the use of immunosuppressive drugs in several immune-mediated conditions. Tolerogenic dendritic cells (tolDCs) regulate antigen-specific tolerance and control the immune response, making them an interesting candidate for cell therapy. Over the last years, different human tolDCs have been generated <i>ex vivo</i>, each with specific phenotypic and functional characteristics. This study aims to highlight the impact of the manufacturing process on tolDC function. For that, two well-known tolDCs, autologous tolerogenic dendritic cells (ATDCs) and interleukin (IL)-10-induced dendritic cells (DC-10s), were compared based on their phenotype, metabolic secretion, transcriptomic profile, and their modulatory function. OurMolecular therapy. Methods & clinical development101605https://www.ebi.ac.uk/biostudies/studies/S-EPMC12589953biostudies-literatureProtocol of generation of tolerogenic dendritic cells affects their transcriptional and metabolic profiles leading to specific tolerogenic functions.PMC12589953Lavercan DChiffoleau ECloteau CCroyal MRouel MSuuring MGregori SRaux ADelhaye TRoyer ALSantoni de Sio FRondeau DMoreau AAmodio GfalseProtocol of generation of tolerogenic dendritic cells affects their transcriptional and metabolic profiles leading to specific tolerogenic functions.Cell therapy is a promising approach for inducing tolerance and thus reducing the use of immunosuppressive drugs in several immune-mediated conditions. Tolerogenic dendritic cells (tolDCs) regulate antigen-specific tolerance and control the immune response, making them an interesting candidate for cell therapy. Over the last years, different human tolDCs have been generated <i>ex vivo</i>, each with specific phenotypic and functional characteristics. This study aims to highlight the impact of the manufacturing process on tolDC function. For that, two well-known tolDCs, autologous tolerogenic dendritic cells (ATDCs) and interleukin (IL)-10-induced dendritic cells (DC-10s), were compared based on their phenotype, metabolic secretion, transcriptomic profile, and their modulatory function. Our2025-01-01T00:00:00Z2025 Dec2026-06-05T14:44:45.623Z2026-05-18T03:08:20.463ZS-EPMC125899534121016910.1016/j.omtm.2025.101605