{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chen B"],"funding":["Hebei Provincial Major Science and Technology Support Plan International Scientific and Technological Cooperation/Hong Kong, Macau, and Taiwan Science and Technology Cooperation Project","CUHK Direct Research Grant","National Natural Science Foundation of China","RGC Postdoctoral Fellowship Scheme","the NSFC-RGC Joint Research Scheme"],"pagination":["282"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12590883"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(1)"],"pubmed_abstract":["Background
Cancer-associated fibroblasts (CAFs) are key regulators of the tumor microenvironment, yet their spatial organization and immunomodulatory functions in H. pylori-associated gastric cancer (GC) remain incompletely understood.Methods
We profiled formalin-fixed paraffin-embedded (FFPE) tumors from 71 GC patients using spatial transcriptomics and integrated single-cell RNA-seq from three independent cohorts (China, USA, and Singapore). CAF-immune cell colocalization was quantified by neighborhood enrichment and aggregation index score. Ligand-receptor inference and trajectory analysis resolved CAF signaling and state transitions. To delineate post-transcriptional control, ARE-motif scanning and expression correlations were combined with laser-assisted crosslinking an"],"journal":["Molecular cancer"],"pubmed_title":["Spatial and functional dissection of cancer-associated fibroblasts-mediated immune modulation in H. pylori-associated gastric cancer."],"pmcid":["PMC12590883"],"funding_grant_id":["N_CUHK448/23","82272990","2024.066","82403017","25297706D","UGC/GEN/562/3"],"pubmed_authors":["Wu J","Zhang F","Liu J","Dong Y","Yu P","Lyu Y","Fang C","Wong CC","Cheung JCT","Yu J","Tang H","To KF","Xie F","Wang S","Cheung AHK","Wang Z","Zheng X","Wu WKK","Kang W","Tong M","Leung KT","Lu C","Burgermeister E","Chen B","Zhang Y","Tian R","Feng T","Xu Y"],"additional_accession":[]},"is_claimable":false,"name":"Spatial and functional dissection of cancer-associated fibroblasts-mediated immune modulation in H. pylori-associated gastric cancer.","description":"Background
Cancer-associated fibroblasts (CAFs) are key regulators of the tumor microenvironment, yet their spatial organization and immunomodulatory functions in H. pylori-associated gastric cancer (GC) remain incompletely understood.Methods
We profiled formalin-fixed paraffin-embedded (FFPE) tumors from 71 GC patients using spatial transcriptomics and integrated single-cell RNA-seq from three independent cohorts (China, USA, and Singapore). CAF-immune cell colocalization was quantified by neighborhood enrichment and aggregation index score. Ligand-receptor inference and trajectory analysis resolved CAF signaling and state transitions. To delineate post-transcriptional control, ARE-motif scanning and expression correlations were combined with laser-assisted crosslinking an","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-10T05:09:17.168Z","creation":"2026-06-10T03:07:37.199Z"},"accession":"S-EPMC12590883","cross_references":{"pubmed":["41194113"],"doi":["10.1186/s12943-025-02490-9"]}}