{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cardenas M"],"funding":["NIAID NIH HHS"],"pagination":["104155"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12593577"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["6(4)"],"pubmed_abstract":["Recent work has shown that major histocompatibility complex (MHC) class II is a functional receptor for some influenza A virus (FLUAV) subtypes. Here, we present a protocol to measure the kinetics of MHC class II-influenza A binding using biolayer interferometry. We describe steps for producing recombinant human leukocyte antigen DR isotype (HLA-DR) MHC class II using a baculovirus expression system. The HLA-DR antigen is then modified for its utilization as a biolayer interferometry (BLI) probe for kinetic experiments using whole FLUAV viruses. This protocol allows for the screening of different FLUAV subtypes and their affinity for MHC class II."],"journal":["STAR protocols"],"pubmed_title":["Protocol to measure the kinetics of MHC class II-influenza A binding using biolayer interferometry."],"pmcid":["PMC12593577"],"funding_grant_id":["75N93021C00014","R01 AI165692"],"pubmed_authors":["Boons GJ","Hunt A","Chopra P","Cardenas M","Rajao DS","Compton S","Perez DR"],"additional_accession":[]},"is_claimable":false,"name":"Protocol to measure the kinetics of MHC class II-influenza A binding using biolayer interferometry.","description":"Recent work has shown that major histocompatibility complex (MHC) class II is a functional receptor for some influenza A virus (FLUAV) subtypes. Here, we present a protocol to measure the kinetics of MHC class II-influenza A binding using biolayer interferometry. We describe steps for producing recombinant human leukocyte antigen DR isotype (HLA-DR) MHC class II using a baculovirus expression system. The HLA-DR antigen is then modified for its utilization as a biolayer interferometry (BLI) probe for kinetic experiments using whole FLUAV viruses. This protocol allows for the screening of different FLUAV subtypes and their affinity for MHC class II.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Oct","modification":"2026-06-05T12:45:38.645Z","creation":"2026-05-17T03:07:44.31Z"},"accession":"S-EPMC12593577","cross_references":{"pubmed":["41138188"],"doi":["10.1016/j.xpro.2025.104155"]}}