{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Teo CF"],"funding":["HHS | NIH | National Institute of Neurological Disorders and Stroke (NINDS)","NINDS NIH HHS","HHS | NIH | National Institute of General Medical Sciences (NIGMS)","HHMI (HHMI)","NIGMS NIH HHS"],"pagination":["e2516822122"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12595458"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["122(44)"],"pubmed_abstract":["The asymmetric resting distribution of the three major phospholipid classes on the mammalian plasma membrane, with phosphatidylserine and phosphatidylethanolamine mostly on the inner leaflet and phosphatidylcholine mostly on the outer leaflet, is maintained by ATP-dependent flippases and floppases that exhibit headgroup selectivity. Upon signaling cues, this asymmetry can be dissipated by various phospholipid scramblases, allowing cells to respond to stimuli and adapt to different physiological contexts. The prevailing view in the field is that phospholipid scramblases on the plasma membrane act without headgroup preference. Here, we report contrary experimental evidence based on a phospholipid scrambling assay that quantifies the fluorescence polarization (FP) of nitrobenzoxadiazole (NBD)"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["A cell-based scrambling assay reveals the phospholipid headgroup preference of TMEM16F on the plasma membrane."],"pmcid":["PMC12595458"],"funding_grant_id":["NA","R01GM137109","R01 GM137109","R35NS137312","R35NS122110","R35 NS122110","R35 NS137312"],"pubmed_authors":["Grabe M","Crottes D","Jan LY","Tuomivaara ST","van Hilten N","Teo CF","Jan YN"],"additional_accession":[]},"is_claimable":false,"name":"A cell-based scrambling assay reveals the phospholipid headgroup preference of TMEM16F on the plasma membrane.","description":"The asymmetric resting distribution of the three major phospholipid classes on the mammalian plasma membrane, with phosphatidylserine and phosphatidylethanolamine mostly on the inner leaflet and phosphatidylcholine mostly on the outer leaflet, is maintained by ATP-dependent flippases and floppases that exhibit headgroup selectivity. Upon signaling cues, this asymmetry can be dissipated by various phospholipid scramblases, allowing cells to respond to stimuli and adapt to different physiological contexts. The prevailing view in the field is that phospholipid scramblases on the plasma membrane act without headgroup preference. Here, we report contrary experimental evidence based on a phospholipid scrambling assay that quantifies the fluorescence polarization (FP) of nitrobenzoxadiazole (NBD)","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-05T12:40:15.826Z","creation":"2026-05-17T03:07:52.405Z"},"accession":"S-EPMC12595458","cross_references":{"pubmed":["41166415"],"doi":["10.1073/pnas.2516822122"]}}