{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kumar R"],"funding":["National Institute of Food and Agriculture"],"pagination":["299"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12596284"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["138(12)"],"pubmed_abstract":["<h4>Key message</h4>A major QTL conferring powdery mildew resistance was fine mapped to a 54.8 kb region on chromosome 2 using an interspecific RIL population (Citrullus mucosospermus × Citrullus lanatus). Four co-segregating KASP markers were developed and validated across multiple populations, demonstrating their utility for marker-assisted selection. Powdery mildew, caused by Podosphaera xanthii, is a major fungal disease that significantly affects watermelon production worldwide. Developing resistant cultivars through marker-assisted selection (MAS) offers an effective and sustainable strategy for disease management. In this study, a 54,772 bp quantitative trait locus (QTL) associated with powdery mildew resistance was mapped to chromosome 2 (30,111,475-30,166,247 bp) using an F<sub>11</sub> recombinant inbred line (RIL) population derived from an interspecific cross between the resistant C. mucosospermus line USVL531-MDR and the susceptible C. lanatus line USVL677-PMS. Genetic analysis revealed that resistance is controlled by a single dominant gene, supported by a 3:1 segregation ratio observed in F<sub>2</sub> populations. The mapped region harbored three lipoxygenase (LOX) genes and one 50S ribosomal protein L27-like gene. Four KASP markers were developed from SNPs located within four putative genes in the QTL region and were validated across multiple segregating populations, including the RIL (USVL531-MDR × USVL677-PMS) and two F<sub>2</sub> populations (USVL531-MDR × 'Sugar Baby' and PI 560003 × USVL677-PMS). These markers accurately differentiated resistant and susceptible individuals (R2 = 0.68-0.82) and exhibited 100% co-segregation with powdery mildew resistance in the RIL and two F<sub>2</sub> populations, demonstrating their utility for MAS. The identified QTL and validated KASP markers will facilitate MAS for powdery mildew resistance breeding and enable future gene cloning work."],"journal":["TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik"],"pubmed_title":["Fine mapping, introgression, and KASP marker development for powdery mildew resistance in watermelon using an interspecific RIL population (Citrullus mucosospermus × C. lanatus)."],"pmcid":["PMC12596284"],"funding_grant_id":["2015-51181-24285","2020-51181-32139"],"pubmed_authors":["Karthikeyan R","Ikerd J","Kumar R","Kousik C"],"additional_accession":[]},"is_claimable":false,"name":"Fine mapping, introgression, and KASP marker development for powdery mildew resistance in watermelon using an interspecific RIL population (Citrullus mucosospermus × C. lanatus).","description":"<h4>Key message</h4>A major QTL conferring powdery mildew resistance was fine mapped to a 54.8 kb region on chromosome 2 using an interspecific RIL population (Citrullus mucosospermus × Citrullus lanatus). Four co-segregating KASP markers were developed and validated across multiple populations, demonstrating their utility for marker-assisted selection. Powdery mildew, caused by Podosphaera xanthii, is a major fungal disease that significantly affects watermelon production worldwide. Developing resistant cultivars through marker-assisted selection (MAS) offers an effective and sustainable strategy for disease management. In this study, a 54,772 bp quantitative trait locus (QTL) associated with powdery mildew resistance was mapped to chromosome 2 (30,111,475-30,166,247 bp) using an F<sub>11</sub> recombinant inbred line (RIL) population derived from an interspecific cross between the resistant C. mucosospermus line USVL531-MDR and the susceptible C. lanatus line USVL677-PMS. Genetic analysis revealed that resistance is controlled by a single dominant gene, supported by a 3:1 segregation ratio observed in F<sub>2</sub> populations. The mapped region harbored three lipoxygenase (LOX) genes and one 50S ribosomal protein L27-like gene. Four KASP markers were developed from SNPs located within four putative genes in the QTL region and were validated across multiple segregating populations, including the RIL (USVL531-MDR × USVL677-PMS) and two F<sub>2</sub> populations (USVL531-MDR × 'Sugar Baby' and PI 560003 × USVL677-PMS). These markers accurately differentiated resistant and susceptible individuals (R2 = 0.68-0.82) and exhibited 100% co-segregation with powdery mildew resistance in the RIL and two F<sub>2</sub> populations, demonstrating their utility for MAS. The identified QTL and validated KASP markers will facilitate MAS for powdery mildew resistance breeding and enable future gene cloning work.","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-06T13:28:16.312Z","creation":"2026-05-31T03:08:03.164Z"},"accession":"S-EPMC12596284","cross_references":{"pubmed":["41205047"],"doi":["10.1007/s00122-025-05079-4"]}}