{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Idris F"],"funding":["PMO | National Research Foundation Singapore (NRF)","MOH | National Medical Research Council (NMRC)"],"pagination":["2995-3020"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12603335"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(11)"],"pubmed_abstract":["Non-structural protein 1 (NS1) of dengue virus (DENV) harbours two conserved N-glycosylation sites at positions 130 and 207, whose biological roles have remained elusive. Using a clinically relevant mouse model of severe dengue, we showed that DENV that lacked N207 glycans on NS1 was significantly attenuated, and this phenotype was dominant over wild-type virulent DENV. Mice infected with this mutant exhibited accelerated viral clearance, milder lymphopenia and more functional DENV-specific CD8<sup>+</sup> T cells. Bulk and single-cell RNA sequencing, cytokine measurements and immune-phenotyping revealed blunted innate inflammatory responses early post-infection, which correlated with reduced PD-L1 expression on innate immune cells and reduced PD-1<sup>+</sup> T-cells in mice infected with"],"journal":["EMBO molecular medicine"],"pubmed_title":["Glycans on non-structural protein 1 prevent premature T-cell mediated dengue virus clearance."],"pmcid":["PMC12603335"],"funding_grant_id":["MOH-000087","NRF-CRP27-2021-0003"],"pubmed_authors":["Copping JM","Alonso S","Allison JR","Idris F","Tan ETX","Li QH","Ooi JSG","Walsh I","Ng LY","Wan C","Marzinek JK","Bond PJ","Chan KR","Torta F","Lim SY","Nguyen-Khuong T","Ting DHR","Benke PI"],"additional_accession":[]},"is_claimable":false,"name":"Glycans on non-structural protein 1 prevent premature T-cell mediated dengue virus clearance.","description":"Non-structural protein 1 (NS1) of dengue virus (DENV) harbours two conserved N-glycosylation sites at positions 130 and 207, whose biological roles have remained elusive. Using a clinically relevant mouse model of severe dengue, we showed that DENV that lacked N207 glycans on NS1 was significantly attenuated, and this phenotype was dominant over wild-type virulent DENV. Mice infected with this mutant exhibited accelerated viral clearance, milder lymphopenia and more functional DENV-specific CD8<sup>+</sup> T cells. Bulk and single-cell RNA sequencing, cytokine measurements and immune-phenotyping revealed blunted innate inflammatory responses early post-infection, which correlated with reduced PD-L1 expression on innate immune cells and reduced PD-1<sup>+</sup> T-cells in mice infected with","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-06T15:45:20.495Z","creation":"2026-06-02T03:09:20.855Z"},"accession":"S-EPMC12603335","cross_references":{"pubmed":["40962941"],"doi":["10.1038/s44321-025-00311-6"]}}