{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ramos-Wolfley AD"],"funding":["NIAID NIH HHS","NIGMS NIH HHS"],"pagination":["5523-5535"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12604032"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(11)"],"pubmed_abstract":["The role of host cell-derived extracellular vesicles (EVs) in host-pathogen interactions remains to be defined during mycobacterial infection. In this study, we characterized EVs from mouse RAW 264.7 cells uninfected or infected with <i>Mycobacterium abscessus</i> (<i>M</i>.<i>ab</i>), one of the most common nontuberculous mycobacterial (NTM) pathogens in humans. Our results show that <i>M</i>.<i>ab</i> infection increased the release of EVs but had no effect on the size distribution and total protein abundance of EVs from RAW 264.7 cells. Interestingly, EVs released by <i>M</i>.<i>ab</i>-infected RAW 264.7 cells significantly enhanced <i>M</i>.<i>ab</i> growth within recipient macrophages in cell culture. The proteomic analysis found that transferrin receptor was enriched in EVs from <i>M"],"journal":["Journal of proteome research"],"pubmed_title":["Host Cell-Derived Extracellular Vesicles Regulate Iron Uptake in Recipient Macrophages during &lt;i&gt;Mycobacterium abscessus&lt;/i&gt; Infection."],"pmcid":["PMC12604032"],"funding_grant_id":["R01 AI173180","P30 GM149368","R21 AI166118"],"pubmed_authors":["Hartson SD","Clark OL","Liu L","Tan X","Zhang J","Ramos-Wolfley AD","Guthrie CM","Speelmon CA","Cheng Y"],"additional_accession":[]},"is_claimable":false,"name":"Host Cell-Derived Extracellular Vesicles Regulate Iron Uptake in Recipient Macrophages during &lt;i&gt;Mycobacterium abscessus&lt;/i&gt; Infection.","description":"The role of host cell-derived extracellular vesicles (EVs) in host-pathogen interactions remains to be defined during mycobacterial infection. In this study, we characterized EVs from mouse RAW 264.7 cells uninfected or infected with <i>Mycobacterium abscessus</i> (<i>M</i>.<i>ab</i>), one of the most common nontuberculous mycobacterial (NTM) pathogens in humans. Our results show that <i>M</i>.<i>ab</i> infection increased the release of EVs but had no effect on the size distribution and total protein abundance of EVs from RAW 264.7 cells. Interestingly, EVs released by <i>M</i>.<i>ab</i>-infected RAW 264.7 cells significantly enhanced <i>M</i>.<i>ab</i> growth within recipient macrophages in cell culture. The proteomic analysis found that transferrin receptor was enriched in EVs from <i>M","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-05T15:03:27.631Z","creation":"2026-05-18T03:12:12.751Z"},"accession":"S-EPMC12604032","cross_references":{"pubmed":["41124677"],"doi":["10.1021/acs.jproteome.5c00399"]}}