biostudies-literatureRamos-Wolfley ADNIAID NIH HHSNIGMS NIH HHS5523-5535https://www.ebi.ac.uk/biostudies/studies/S-EPMC12604032biostudies-literatureUnknown24(11)The role of host cell-derived extracellular vesicles (EVs) in host-pathogen interactions remains to be defined during mycobacterial infection. In this study, we characterized EVs from mouse RAW 264.7 cells uninfected or infected with <i>Mycobacterium abscessus</i> (<i>M</i>.<i>ab</i>), one of the most common nontuberculous mycobacterial (NTM) pathogens in humans. Our results show that <i>M</i>.<i>ab</i> infection increased the release of EVs but had no effect on the size distribution and total protein abundance of EVs from RAW 264.7 cells. Interestingly, EVs released by <i>M</i>.<i>ab</i>-infected RAW 264.7 cells significantly enhanced <i>M</i>.<i>ab</i> growth within recipient macrophages in cell culture. The proteomic analysis found that transferrin receptor was enriched in EVs from <i>MJournal of proteome researchHost Cell-Derived Extracellular Vesicles Regulate Iron Uptake in Recipient Macrophages during <i>Mycobacterium abscessus</i> Infection.PMC12604032R01 AI173180P30 GM149368R21 AI166118Hartson SDClark OLLiu LTan XZhang JRamos-Wolfley ADGuthrie CMSpeelmon CACheng YfalseHost Cell-Derived Extracellular Vesicles Regulate Iron Uptake in Recipient Macrophages during <i>Mycobacterium abscessus</i> Infection.The role of host cell-derived extracellular vesicles (EVs) in host-pathogen interactions remains to be defined during mycobacterial infection. In this study, we characterized EVs from mouse RAW 264.7 cells uninfected or infected with <i>Mycobacterium abscessus</i> (<i>M</i>.<i>ab</i>), one of the most common nontuberculous mycobacterial (NTM) pathogens in humans. Our results show that <i>M</i>.<i>ab</i> infection increased the release of EVs but had no effect on the size distribution and total protein abundance of EVs from RAW 264.7 cells. Interestingly, EVs released by <i>M</i>.<i>ab</i>-infected RAW 264.7 cells significantly enhanced <i>M</i>.<i>ab</i> growth within recipient macrophages in cell culture. The proteomic analysis found that transferrin receptor was enriched in EVs from <i>M2025-01-01T00:00:00Z2025 Nov2026-06-05T15:03:27.631Z2026-05-18T03:12:12.751ZS-EPMC126040324112467710.1021/acs.jproteome.5c00399