{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Baros-Steyl SS"],"funding":["University of Capetown","South African Medical Research Council","National Research Foundation"],"pagination":["5585-5603"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12604046"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(11)"],"pubmed_abstract":["Pathogenic mycobacteria, such as <i>Mycobacterium tuberculosis</i>, modulate the host immune system to evade clearance and promote long-term persistence, leading to disease progression or latent infection. Understanding how these mycobacteria evade elimination is key to uncovering the molecular mechanisms of infection. Protein kinase G (PknG) in pathogenic mycobacteria plays a critical role in avoiding macrophage clearance by inhibiting phagosome-lysosome fusion; however, the exact mechanism is not completely understood. To investigate the role of PknG during early events of macrophage infection, RAW 264.7 macrophages were infected with <i>Mycobacterium bovis</i> BCG wild-type and PknG knockout mutant strains. Phosphoproteomic analysis, including TiO<sub>2</sub>-based phosphopeptide enrich"],"journal":["Journal of proteome research"],"pubmed_title":["A Phosphoproteomic Analysis of Mycobacterial PknG-Mediated Host Immune Evasion."],"pmcid":["PMC12604046"],"funding_grant_id":["95984","467126"],"pubmed_authors":["Soares NC","Ganief TA","Blackburn JM","Nakedi KC","Baros-Steyl SS"],"additional_accession":[]},"is_claimable":false,"name":"A Phosphoproteomic Analysis of Mycobacterial PknG-Mediated Host Immune Evasion.","description":"Pathogenic mycobacteria, such as <i>Mycobacterium tuberculosis</i>, modulate the host immune system to evade clearance and promote long-term persistence, leading to disease progression or latent infection. Understanding how these mycobacteria evade elimination is key to uncovering the molecular mechanisms of infection. Protein kinase G (PknG) in pathogenic mycobacteria plays a critical role in avoiding macrophage clearance by inhibiting phagosome-lysosome fusion; however, the exact mechanism is not completely understood. To investigate the role of PknG during early events of macrophage infection, RAW 264.7 macrophages were infected with <i>Mycobacterium bovis</i> BCG wild-type and PknG knockout mutant strains. Phosphoproteomic analysis, including TiO<sub>2</sub>-based phosphopeptide enrich","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-05T12:49:57.127Z","creation":"2026-05-17T03:07:51.949Z"},"accession":"S-EPMC12604046","cross_references":{"pubmed":["41064949"],"doi":["10.1021/acs.jproteome.5c00416"]}}