biostudies-literatureBaros-Steyl SSUniversity of CapetownSouth African Medical Research CouncilNational Research Foundation5585-5603https://www.ebi.ac.uk/biostudies/studies/S-EPMC12604046biostudies-literatureUnknown24(11)Pathogenic mycobacteria, such as <i>Mycobacterium tuberculosis</i>, modulate the host immune system to evade clearance and promote long-term persistence, leading to disease progression or latent infection. Understanding how these mycobacteria evade elimination is key to uncovering the molecular mechanisms of infection. Protein kinase G (PknG) in pathogenic mycobacteria plays a critical role in avoiding macrophage clearance by inhibiting phagosome-lysosome fusion; however, the exact mechanism is not completely understood. To investigate the role of PknG during early events of macrophage infection, RAW 264.7 macrophages were infected with <i>Mycobacterium bovis</i> BCG wild-type and PknG knockout mutant strains. Phosphoproteomic analysis, including TiO₂-based phosphopeptide enrichJournal of proteome researchA Phosphoproteomic Analysis of Mycobacterial PknG-Mediated Host Immune Evasion.PMC1260404695984467126Soares NCGanief TABlackburn JMNakedi KCBaros-Steyl SSfalseA Phosphoproteomic Analysis of Mycobacterial PknG-Mediated Host Immune Evasion.Pathogenic mycobacteria, such as <i>Mycobacterium tuberculosis</i>, modulate the host immune system to evade clearance and promote long-term persistence, leading to disease progression or latent infection. Understanding how these mycobacteria evade elimination is key to uncovering the molecular mechanisms of infection. Protein kinase G (PknG) in pathogenic mycobacteria plays a critical role in avoiding macrophage clearance by inhibiting phagosome-lysosome fusion; however, the exact mechanism is not completely understood. To investigate the role of PknG during early events of macrophage infection, RAW 264.7 macrophages were infected with <i>Mycobacterium bovis</i> BCG wild-type and PknG knockout mutant strains. Phosphoproteomic analysis, including TiO₂-based phosphopeptide enrich2025-01-01T00:00:00Z2025 Nov2026-06-05T12:49:57.127Z2026-05-17T03:07:51.949ZS-EPMC126040464106494910.1021/acs.jproteome.5c00416