{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chen KZ"],"funding":["National Natural Science Foundation of China"],"pagination":["78"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12604166"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(1)"],"pubmed_abstract":["<h4>Background</h4>Tenascin-C (TNC) is an extracellular matrix (ECM) protein involved in tissue damage and fibrosis. Chimeric antigen receptor (CAR) cell therapy is a novel therapeutic approach that has attracted increasing attention in recent years. Here, we engineered CAR-macrophages targeting TNC (TNC-CAR-Ms) and explored the underlying mechanism through which TNC-CAR-Ms treat liver fibrosis.<h4>Methods</h4>The role of TNC in liver fibrosis was studied in established Tnc knockout (KO) and littermate control mice. A TNC-targeted single-chain variable fragment (scFv) was designed to generate TNC-CAR-Ms and evaluate their biological function. The phagocytosis and killing effects of TNC-CAR-Ms were tested in vitro, while the antifibrotic efficacy and safety of TNC-CAR-Ms were evaluated in v"],"journal":["Military Medical Research"],"pubmed_title":["TNC-targeted CAR-macrophage therapy alleviates liver fibrosis in mice."],"pmcid":["PMC12604166"],"funding_grant_id":["82204500","82072697"],"pubmed_authors":["Zhang W","Huo YK","Zhou YX","Fu Q","Ma XD","Zhang SS","Wan HY","Gao ZQ","Lin ZY","Chen KZ","Chen LJ","Cheng HW"],"additional_accession":[]},"is_claimable":false,"name":"TNC-targeted CAR-macrophage therapy alleviates liver fibrosis in mice.","description":"<h4>Background</h4>Tenascin-C (TNC) is an extracellular matrix (ECM) protein involved in tissue damage and fibrosis. Chimeric antigen receptor (CAR) cell therapy is a novel therapeutic approach that has attracted increasing attention in recent years. Here, we engineered CAR-macrophages targeting TNC (TNC-CAR-Ms) and explored the underlying mechanism through which TNC-CAR-Ms treat liver fibrosis.<h4>Methods</h4>The role of TNC in liver fibrosis was studied in established Tnc knockout (KO) and littermate control mice. A TNC-targeted single-chain variable fragment (scFv) was designed to generate TNC-CAR-Ms and evaluate their biological function. The phagocytosis and killing effects of TNC-CAR-Ms were tested in vitro, while the antifibrotic efficacy and safety of TNC-CAR-Ms were evaluated in v","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-05T12:52:39.37Z","creation":"2026-05-17T03:07:53.479Z"},"accession":"S-EPMC12604166","cross_references":{"pubmed":["41214839"],"doi":["10.1186/s40779-025-00667-3"]}}