{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["25(1)"],"submitter":["Su YS"],"pubmed_abstract":["<h4>Background</h4>Rapid and accurate viral detection is essential for clinical diagnosis and effective outbreak surveillance. Traditional methods, including culture-based isolation and antigen tests, are time-consuming and limited by tissue tropism. Multiplex PCR panels, although faster, are constrained by predefined targets, limiting their ability to detect novel or unexpected viral strains.<h4>Methods</h4>We applied Oxford Nanopore Technology sequencing (ONT-Seq), a long-read, real-time, and multiplex metagenomic platform, to 85 clinical specimens using a sequence-independent, single-primer amplification (SISPA) workflow. Sequencing results were compared with routine clinical diagnostics for concordance and for identification of co-infections RESULTS: ONT-Seq achieved 80% concordance wi"],"journal":["BMC infectious diseases"],"pagination":["1531"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12604265"],"repository":["biostudies-literature"],"pubmed_title":["Multiplex metagenomic sequencing for rapid viral pathogen identification and surveillance in clinical specimens."],"pmcid":["PMC12604265"],"pubmed_authors":["Jiang NR","Wu HC","Tsai WH","Lee CY","Chi CY","Kuo YP","Chen FJ","Yu GY","Liao YC","Cheng SH","Huang CT","Tsai WT","Lee KL","Chiu YT","Lin EJ","Su YS","Tien CF"],"additional_accession":[]},"is_claimable":false,"name":"Multiplex metagenomic sequencing for rapid viral pathogen identification and surveillance in clinical specimens.","description":"<h4>Background</h4>Rapid and accurate viral detection is essential for clinical diagnosis and effective outbreak surveillance. Traditional methods, including culture-based isolation and antigen tests, are time-consuming and limited by tissue tropism. Multiplex PCR panels, although faster, are constrained by predefined targets, limiting their ability to detect novel or unexpected viral strains.<h4>Methods</h4>We applied Oxford Nanopore Technology sequencing (ONT-Seq), a long-read, real-time, and multiplex metagenomic platform, to 85 clinical specimens using a sequence-independent, single-primer amplification (SISPA) workflow. Sequencing results were compared with routine clinical diagnostics for concordance and for identification of co-infections RESULTS: ONT-Seq achieved 80% concordance wi","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-07-15T13:32:02.064Z","creation":"2026-07-05T03:08:40.465Z"},"accession":"S-EPMC12604265","cross_references":{"pubmed":["41214546"],"doi":["10.1186/s12879-025-11952-w"]}}