{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ludin A"],"funding":["NCI NIH HHS","NINDS NIH HHS"],"pagination":["6720-6736.e26"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC12604482"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["188(24)"],"pubmed_abstract":["T cell-mediated tumor killing underlies immunotherapy success. Here, we used long-term in vivo imaging and high-resolution spatial transcriptomics of zebrafish endogenous melanoma, as well as multiplex imaging of human melanoma, to identify domains facilitating the immune response during immunotherapy. We identified cancer regions of antigen presentation and T cell engagement and retention (CRATERs) as pockets at the stroma-melanocyte boundaries of zebrafish and human melanoma. CRATERs are rich in antigen-recognition molecules, harboring the highest density of CD8<sup>+</sup> T cells in tumors. In zebrafish, CD8<sup>+</sup> T cells formed prolonged interactions with melanoma cells within CRATERs, characteristic of antigen recognition. Following immunostimulatory treatment, CRATERs expanded"],"journal":["Cell"],"pubmed_title":["CRATER tumor niches facilitate CD8&lt;sup&gt;+&lt;/sup&gt; T cell engagement and correspond with immunotherapy success."],"pmcid":["PMC12604482"],"funding_grant_id":["P01 CA163222","P30 NS072030","F31 CA260802"],"pubmed_authors":["Cordova M","Manos M","Wu L","Gong Q","Deng L","Rajadhyaksha M","Liu S","Alessi JV","Weiss JD","Eskndir N","Ludin A","Stirtz GL","Hodi FS","Rodig SJ","Murphy GF","Jones SM","Sorger PK","Zon LI","Pessoa Rodrigues C","Stanhope ME","Chen F","Afeyan AB","Nirmal AJ","Zhou Y","Tal A","McCall WD","Awad MM","Formato E","Ricciuti B","Sefton J","Wucherpfennig KW","Barrera I","Cole HL","Faulkner HM","Sahu A","Besson N","Moreau HM","Quake SR","Hoytema van Konijnenburg DP","Porter B","Pfaff KL","Yang S","Jerison E","Richardson DS"],"additional_accession":[]},"is_claimable":false,"name":"CRATER tumor niches facilitate CD8&lt;sup&gt;+&lt;/sup&gt; T cell engagement and correspond with immunotherapy success.","description":"T cell-mediated tumor killing underlies immunotherapy success. Here, we used long-term in vivo imaging and high-resolution spatial transcriptomics of zebrafish endogenous melanoma, as well as multiplex imaging of human melanoma, to identify domains facilitating the immune response during immunotherapy. We identified cancer regions of antigen presentation and T cell engagement and retention (CRATERs) as pockets at the stroma-melanocyte boundaries of zebrafish and human melanoma. CRATERs are rich in antigen-recognition molecules, harboring the highest density of CD8<sup>+</sup> T cells in tumors. In zebrafish, CD8<sup>+</sup> T cells formed prolonged interactions with melanoma cells within CRATERs, characteristic of antigen recognition. Following immunostimulatory treatment, CRATERs expanded","dates":{"release":"2025-01-01T00:00:00Z","publication":"2025 Nov","modification":"2026-06-06T17:07:13.667Z","creation":"2026-06-02T03:12:03.672Z"},"accession":"S-EPMC12604482","cross_references":{"pubmed":["41109214"],"doi":["10.1016/j.cell.2025.09.021"]}}